CAJ | 학술논문

目的:建立有瓜石斛中原儿茶酸的含量测定方法.方法:采用高效液相色谱(HPLC)法.色谱柱为Zorbax SB-Aq色谱柱;流动相为乙腈-0.1%磷酸溶液;检测波长为220nm;流速为1.0mL·min-1;柱温为30℃. 结果:原儿茶酸达到基线分离,线性关系良好(r=1.0000);平均加样回收率为101.71%,RSD为2.48%(n=6). 12批市售样品含量在0.0374~0.203mg·g-1范围内,差异相对较大. 结论:该方法灵敏,可靠,可用作有瓜石斛药材的质量评价指标之一.
목적:건립유과석곡중원인다산적함량측정방법.방법:채용고효액상색보(HPLC)법.색보주위Zorbax SB-Aq색보주;류동상위을정-0.1%린산용액;검측파장위220nm;류속위1.0mL·min-1;주온위30℃. 결과:원인다산체도기선분리,선성관계량호(r=1.0000);평균가양회수솔위101.71%,RSD위2.48%(n=6). 12비시수양품함량재0.0374~0.203mg·g-1범위내,차이상대교대. 결론:해방법령민,가고,가용작유과석곡약재적질량평개지표지일.
Objective:To establish a method for determintation of protocatechuic acid in Herba flickingeriae by HPLC. Methods:HPLC method was used with the Zorbax SB-Aq column, the acetonitrile-0.1% phosphoric acid was employed as a mobile phase, detection wavelength was 220nm. Column temperature was 30℃, and flow rate was 1.0mL·min-1. Results:Baseline separation was obtained and linear relationship was good (r=1.0000) for protocatechuic acid content. The average recovery was 101.71%and RSD was 2.48%(n=6). The content of protocatechuic acid in the 12 samples of commercially available Herba flickingeriae were among 0.0374~0.203mg·g-1, with distinct difference. Conclusion:The method is sensitive, reliable and available for the quality control of Herba flickingeriae.