中国医药
中國醫藥
중국의약
China Medicine
2015年
10期
1517-1520
,共4页
马春华%夏小艳%玛依努尔·尼牙孜%韩莉莉%朱开春
馬春華%夏小豔%瑪依努爾·尼牙孜%韓莉莉%硃開春
마춘화%하소염%마의노이·니아자%한리리%주개춘
宫颈鳞癌%人类白细胞抗原DQB1%易感基因%聚合酶链反应-直接测序分型
宮頸鱗癌%人類白細胞抗原DQB1%易感基因%聚閤酶鏈反應-直接測序分型
궁경린암%인류백세포항원DQB1%역감기인%취합매련반응-직접측서분형
Cervical squamous cancer%Human leucocyte antigen-DQB1%Predisposing genes%Polymerase chain reaction-sequence-based typing
目的 探讨新疆维吾尔族妇女宫颈鳞癌与人类白细胞抗原(HLA) DQB1基因多态性的相关性.方法 采用聚合酶链反应-直接测序分型法对80例于2009年8月至2010年4月在新疆维吾尔自治区人民医院就诊的新疆维吾尔族妇女宫颈鳞癌组织(观察组)及80例新疆维吾尔族妇女宫颈炎性组织(对照组)进行HLA-DQB1基因分型.比较2组HLA-DQB1等位基因及基因型频率,计算等位基因的比值比(OR)和95％置信区间(CI),用以估计等位基因与疾病联系的程度.结果 检测的160个样本中共含296个等位基因,其中杂合的等位基因型136人份,纯合的等位基因型24人份.经过频率计算和统计学分析发现,观察组HLA-DQB1* 0325(OR:10.60,95％ CI:1.341～83.810)和HLA-DQB1* 0332(OR:12.59,95％ CI:2.909～ 54.526)等位基因频率明显高于对照组,而HLA-DQB1女0317(OR:0.49,95％ CI:0.304～0.798)和HLA-DQB1* 040302(OR:0.40,95％ CI:0.243 ～0.658)等位基因频率明显低于对照组,差异均有统计学意义(均P＜0.05).结论 新疆维吾尔族妇女宫颈鳞癌中HLA-DQB1基因型与我国其他地区报道有差异,等位基因HLA-DQB1*0325和HLA-DQB1* 0332可能是新疆维吾尔族妇女宫颈鳞癌的易感基因,而HLA-DQB1 * 0317和HLA-DQB1*040302可能是新疆维吾尔族妇女宫颈鳞癌的保护基因.
目的 探討新疆維吾爾族婦女宮頸鱗癌與人類白細胞抗原(HLA) DQB1基因多態性的相關性.方法 採用聚閤酶鏈反應-直接測序分型法對80例于2009年8月至2010年4月在新疆維吾爾自治區人民醫院就診的新疆維吾爾族婦女宮頸鱗癌組織(觀察組)及80例新疆維吾爾族婦女宮頸炎性組織(對照組)進行HLA-DQB1基因分型.比較2組HLA-DQB1等位基因及基因型頻率,計算等位基因的比值比(OR)和95％置信區間(CI),用以估計等位基因與疾病聯繫的程度.結果 檢測的160箇樣本中共含296箇等位基因,其中雜閤的等位基因型136人份,純閤的等位基因型24人份.經過頻率計算和統計學分析髮現,觀察組HLA-DQB1* 0325(OR:10.60,95％ CI:1.341～83.810)和HLA-DQB1* 0332(OR:12.59,95％ CI:2.909～ 54.526)等位基因頻率明顯高于對照組,而HLA-DQB1女0317(OR:0.49,95％ CI:0.304～0.798)和HLA-DQB1* 040302(OR:0.40,95％ CI:0.243 ～0.658)等位基因頻率明顯低于對照組,差異均有統計學意義(均P＜0.05).結論 新疆維吾爾族婦女宮頸鱗癌中HLA-DQB1基因型與我國其他地區報道有差異,等位基因HLA-DQB1*0325和HLA-DQB1* 0332可能是新疆維吾爾族婦女宮頸鱗癌的易感基因,而HLA-DQB1 * 0317和HLA-DQB1*040302可能是新疆維吾爾族婦女宮頸鱗癌的保護基因.
목적 탐토신강유오이족부녀궁경린암여인류백세포항원(HLA) DQB1기인다태성적상관성.방법 채용취합매련반응-직접측서분형법대80례우2009년8월지2010년4월재신강유오이자치구인민의원취진적신강유오이족부녀궁경린암조직(관찰조)급80례신강유오이족부녀궁경염성조직(대조조)진행HLA-DQB1기인분형.비교2조HLA-DQB1등위기인급기인형빈솔,계산등위기인적비치비(OR)화95％치신구간(CI),용이고계등위기인여질병련계적정도.결과 검측적160개양본중공함296개등위기인,기중잡합적등위기인형136인빈,순합적등위기인형24인빈.경과빈솔계산화통계학분석발현,관찰조HLA-DQB1* 0325(OR:10.60,95％ CI:1.341～83.810)화HLA-DQB1* 0332(OR:12.59,95％ CI:2.909～ 54.526)등위기인빈솔명현고우대조조,이HLA-DQB1녀0317(OR:0.49,95％ CI:0.304～0.798)화HLA-DQB1* 040302(OR:0.40,95％ CI:0.243 ～0.658)등위기인빈솔명현저우대조조,차이균유통계학의의(균P＜0.05).결론 신강유오이족부녀궁경린암중HLA-DQB1기인형여아국기타지구보도유차이,등위기인HLA-DQB1*0325화HLA-DQB1* 0332가능시신강유오이족부녀궁경린암적역감기인,이HLA-DQB1 * 0317화HLA-DQB1*040302가능시신강유오이족부녀궁경린암적보호기인.
Objective To investigate the correlation between human leucocyte antigen-DQB1 allele polymorphism and cervical squamous carcinoma in Uygur women.Methods The cervix tissue from 80 cases of cervical squamous carcinoma (observation group) and 80 cases of cervicitis (control group) from August 2009 to April 2010 were collected;polymerase chain reaction-sequencing based typing method was used to determine the HLA-DQB1 allele polymorphism.The allele type and frequency of allele types were compared between the two groups and the correlation between allele polymorphism with cervical squamous carcinoma was analyzed.Results Two hundred and ninety-six alleles were found in the 160 cases;among them 136 cases had heterozygous alleles and 24 cases had homozygous alleles.The frequencies of HLA-DQB1 * 0325 (OR:10.60,95％ CI:1.341-83.810) and HLA-DQB1 * 0332 (OR:12.59,95％ CI:2.909-54.526) in observation group were significantly higher than those in control group (P ＜ 0.05),but HLA-DQB1 * 0317 (OR:0.49,95％ CI:0.304-0.798) and HLA-DQB1 * 040302 (OR:0.40,95％ CI:0.243-0.658) in observation group were lower thanthose in control group (P ＜ 0.05).Conclusion The genotype of HLA-DQB1 in Uygur women is different from that in others;HLA-DQB1 * 0325 and HLA-DQB1 * 0332 may be the predisposing genes,meanwhile HLA-DQB1 *0317 and HLA-DQB1 * 040302 may be the protective genes.
