CAJ | 학술논문

目的探究乳腺癌患者术后腋窝引流液中细胞角蛋白(CK)19 mRNA的水平及临床意义.方法选取2012年1月至2014年1月蚌埠医学院第一附属医院收治的90例女性乳腺癌患者(乳腺癌组),所有患者均行乳腺癌改良根治术,同时选取50例女性乳腺良性病变的患者作为对照组,采集2组患者术后第2天腋窝引流液,提取引流液mRNA,采用实时聚合酶链反应检测并比较2组患者CK-19 mRNA水平,分析不同乳腺癌病理分期、淋巴结转移、肿瘤大小与CK-19 mRNA阳性的关系.结果乳腺癌组患者CK-19 mRNA水平明显高于对照组,差异有统计学意义[(7.6±3.5)比(1.0±0.5),P＜0.05].乳腺癌组患者32例CK-19 mRNA阳性,阳性率明显高于对照组[35.6％ (32/90)比0.0％ (0/50)],差异有统计学意义(P＜0.05).乳腺癌患者随着病理分期的加重,CK-19 mRNA阳性率升高,Ⅰ、Ⅱ、Ⅲ期分别为19.4％(7/36)、35.5％(11/31)、60.9％(14/23),两两比较差异均有统计学意义(P＜0.05).腋窝淋巴结转移阳性患者CK-19 mRNA阳性率明显高于腋窝淋巴结转移阴性患者,差异有统计学意义[52.2％(24/46)比18.2％ (8/44),P＜0.05].随着乳腺癌患者肿瘤病灶的增大,CK-19 mRNA阳性率逐渐升高,肿瘤直径≤2 cm、＞2 cm且≤5 cm、＞5 cm CK-19 mRNA阳性率分别为15.4％(2/13)、28.8％(15/52)、60.0％(15/25),两两比较差异均有统计学意义(P＜0.05).结论乳腺癌患者术后腋窝引流液CK-19 mRNA水平明显升高,且与病理分期、肿瘤大小、淋巴结转移有相关性;CK-19 mRNA在腋窝引流液中的水平可以作为评判预后的重要指标.
목적 탐구유선암환자술후액와인류액중세포각단백(CK)19 mRNA적수평급림상의의.방법 선취2012년1월지2014년1월방부의학원제일부속의원수치적90례녀성유선암환자(유선암조),소유환자균행유선암개량근치술,동시선취50례녀성유선량성병변적환자작위대조조,채집2조환자술후제2천액와인류액,제취인류액mRNA,채용실시취합매련반응검측병비교2조환자CK-19 mRNA수평,분석불동유선암병리분기、림파결전이、종류대소여CK-19 mRNA양성적관계.결과 유선암조환자CK-19 mRNA수평명현고우대조조,차이유통계학의의[(7.6±3.5)비(1.0±0.5),P＜0.05].유선암조환자32례CK-19 mRNA양성,양성솔명현고우대조조[35.6％ (32/90)비0.0％ (0/50)],차이유통계학의의(P＜0.05).유선암환자수착병리분기적가중,CK-19 mRNA양성솔승고,Ⅰ、Ⅱ、Ⅲ기분별위19.4％(7/36)、35.5％(11/31)、60.9％(14/23),량량비교차이균유통계학의의(P＜0.05).액와림파결전이양성환자CK-19 mRNA양성솔명현고우액와림파결전이음성환자,차이유통계학의의[52.2％(24/46)비18.2％ (8/44),P＜0.05].수착유선암환자종류병조적증대,CK-19 mRNA양성솔축점승고,종류직경≤2 cm、＞2 cm차≤5 cm、＞5 cm CK-19 mRNA양성솔분별위15.4％(2/13)、28.8％(15/52)、60.0％(15/25),량량비교차이균유통계학의의(P＜0.05).결론 유선암환자술후액와인류액CK-19 mRNA수평명현승고,차여병리분기、종류대소、림파결전이유상관성;CK-19 mRNA재액와인류액중적수평가이작위평판예후적중요지표.
Objective To explore the clinical significance of cytokeratin-19 mRNA in postoperative axillary drainage in breast cancer patients.Methods Totally 90 female patients with breast cancer who underwent modified radical mastectomy from January 2012 to January 2014 were enrolled (breast cancer group);50 patients with benign breast lesions were set as a control group.The axillary drainage 2 days after operation was collected and real time-polymerase chain reaction was used to detect the level of CK-19 mRNA in the drainage.The level of CK-19 mRNA was compared between the two groups and the correlations of CK-19 mRNA level with pathology stage,lymph node metastasis and tumor size were analyzed.Results The level of CK-19 mRNA in breast cancer patients was significantly higher than that in control group [(7.6 ± 3.5) vs (1.0 ± 0.5)] (P ＜ 0.05);the positive rate of CK-19 mRNA in breast cancer group was significantly higher than that in control group [35.6％ (32/90) vs 0.0％ (0/50)] (P ＜ 0.05).The positive rate of CK-1 9 mRNA was increased along with pathological stage [stage Ⅰ ∶ 19.4％ (7/36),stage Ⅱ ∶ 60.9％ (14/23),stage Ⅲ ∶ 60.9％ (14/23)] (P ＜ 0.05);the positive rate of CK-19 mRNA in patients with axillary lymph node metastasis was significantly higher than that in patients without axillary lymph node metastasis [52.2％ (24/46) vs 18.2％ (8/44)] (P ＜0.05);the positive rate of CK-19 mRNA was increased with increase of the tumor size [diameter ≤2 cm:15.4％ (2/13),2 cm ＜ diameter ≤5 cm:28.8％ (15/52),diameter ＞5 cm:60.0％ (15/25)] (P＜0.05).Conclusion The level of CK-19 mRNA in postoperative axillary drainage in breast cancer patients is increased and related to pathologic stage,tumor size and axillary lymph node metastasis;the level of CK-19 mRNA can be used as a indicator to assess the prognosis.