CAJ | 학술논문

利用乙二胺(EDA)对聚甲基丙烯酸缩水甘油酯(PGMA)进行开环反应,制备了侧链多氨基聚合物PGMA-EDA；再利用聚乙二醇(PEG-COOH)和硫酸葡聚糖钠盐(DS)分别对PGMA-EDA上氨基进行酰胺化反应和还原胺化反应,制备含动脉粥样硬化斑块靶向分子DS的双亲性接枝共聚物PGMA-EDA-g-PEG-g-DS.通过核磁共振(1HNMR)谱和红外光谱(FTIR)表征了聚合物的结构.利用凝胶渗透色谱(GPC)表征了聚合物的数均分子量Mn=16255,多分散性指数PDI=1.54.采用配体交换法,利用该聚合物对油胺配体超顺磁性氧化铁纳米粒子进行修饰,制备了水溶性氧化铁纳米粒子PGMA-EDA-g-PEG-g-DS@IO.通过透射电镜(TEM)和动态光散射(DLS)表征了纳米粒子的形貌和粒度,采用热重分析(TGA)和振动样品磁强(VSM)仪表征了纳米粒子的包覆率和磁强度.采用细胞计数试剂盒(CCK)测定了纳米粒子的细胞毒性,结果表明,水溶性纳米粒子的生物相容性较好,可作为动脉粥样硬化斑块的特异性磁共振检测用造影剂.
이용을이알(EDA)대취갑기병희산축수감유지(PGMA)진행개배반응,제비료측련다안기취합물PGMA-EDA；재이용취을이순(PEG-COOH)화류산포취당납염(DS)분별대PGMA-EDA상안기진행선알화반응화환원알화반응,제비함동맥죽양경화반괴파향분자DS적쌍친성접지공취물PGMA-EDA-g-PEG-g-DS.통과핵자공진(1HNMR)보화홍외광보(FTIR)표정료취합물적결구.이용응효삼투색보(GPC)표정료취합물적수균분자량Mn=16255,다분산성지수PDI=1.54.채용배체교환법,이용해취합물대유알배체초순자성양화철납미입자진행수식,제비료수용성양화철납미입자PGMA-EDA-g-PEG-g-DS@IO.통과투사전경(TEM)화동태광산사(DLS)표정료납미입자적형모화립도,채용열중분석(TGA)화진동양품자강(VSM)의표정료납미입자적포복솔화자강도.채용세포계수시제합(CCK)측정료납미입자적세포독성,결과표명,수용성납미입자적생물상용성교호,가작위동맥죽양경화반괴적특이성자공진검측용조영제.
To design a new magnetic resonance(MR) imaging contrast agent of atherosclerosis, poly(glycidyl methacrylate)-graft-ethane diamine ( PGMA-g-EDA) was prepared by the ring-opening reaction of PGMA which was synthesized by atom transfer radical polymerization ( ATRP). Poly-ethylene glycol ( PEG) and dextran sulfate(DS) were graft modified to PGMA-g-EDA continuously via amidation reaction and reductive amination to synthesis PGMA-EDA-g-PEG-g-DS. The structure and properties of PGMA-EDA-g-PEG-g-DS were characterized through nuclear magnetic resonance( 1 H NMR). Fourier transform infrared spectroscopy (FTIR) and gel permeation chromatography(GPC). The superparamagnetic iron oxide nanoparticles(IONPs) were coated with PGMA-EDA-g-PEG-g-DS by ligand exchange to prepare water-soluble IO. The transmission electron microscopy( TEM) analysis indicated that micelles were well dispersed in water and had uniform sizes. The result of thermogravimetric analysis(TGA) indicated that about 70% (mass fraction) polymers coa-ted on the surface of IO. The cell counting kit(CCK) assay showed no significant toxicity to RAW264. 7. The above results confirm that PGMA-EDA-g-PEG-g-DS @ IO can be used as a potential contrast agent for atherosclerosis MR imaging.