CAJ | 학술논문

依据子实体的形态特征、培养特性，对采自黑龙江省大兴安岭呼中国家级自然保护区的野生药用真菌进行了鉴定。结果表明：该菌PCR扩增产物长761 bp。 ITS序列聚类结果表明，该菌种与Inonotus obliquus isolate FS656163以及Inonotus obliquus strain MDJCBS88同源性最近，为桦褐孔菌的不同菌株，而与 Inonotus linteus strain PL08121和Inonotu s rikci i isolate PF241亲缘性最远，都属于纤孔菌属。结合分子生物学与形态学鉴定，一致确定其为桦褐孔菌（ Inonout s obilq uus）。
의거자실체적형태특정、배양특성，대채자흑룡강성대흥안령호중국가급자연보호구적야생약용진균진행료감정。결과표명：해균PCR확증산물장761 bp。 ITS서렬취류결과표명，해균충여Inonotus obliquus isolate FS656163이급Inonotus obliquus strain MDJCBS88동원성최근，위화갈공균적불동균주，이여 Inonotus linteus strain PL08121화Inonotu s rikci i isolate PF241친연성최원，도속우섬공균속。결합분자생물학여형태학감정，일치학정기위화갈공균（ Inonout s obilq uus）。
On the basis of the fruiting body morphology and its cultural characteristics , we identified the wild medicinal fungi collected from Daxing ’ an Mountains Huzhong National Nature Reserve of Heilongjiang Province .The length of the PCR product was 761 bp.We took the phylogenetic analysis of the ITS region for Inonotus.The phenogram inferred from the align-ment showed that I.obliquus was evolutionarily closely related to isolate FS 656163 and strain MDJCBS88, and evolutionarily the furthest related to I.linteus strain PL08121 and I.rickii isolate PF241.By the molecular and morphological identifica -tion, we identified the species as I. obliqusu.