CAJ | 학술논문

目的：研究丙型肝炎病毒（HCV）携带者病毒基因型流行趋势和分布特征，探讨其临床应用价值。方法：随机收集无临床症状 HCV携带者血清样本共计90例，HCVRNA含量大于1×103 IU／ml ，其中男58例，女32例。应用聚合酶链式反应（PCR）和反向斑点杂交技术（RDB）进行HCV基因分型检测，并对受检者相关资料进行分析。结果：HCV携带者 HCVRNA 平均浓度为4．94±1．36 lg IU／ml。不同性别组之间 HCVRNA 含量无明显差异（P＞0．05）。所有标本HCV基因成功分型。单基因型感染为主要感染类型，占95．56％（86／90）。其中1b型感染率为48．89％（44／90），2a型感染率为42．22（38／90）。不同性别组 HCV携带者 HCV基因型分布差异无统计学意义（P＞0．05）。结论：本地区 HCV携带者感染主要类型为单基因型感染；HCV1b为主要基因型，其次为2a型；开展HCV基因型检测具有重要的临床意义。
목적：연구병형간염병독（HCV）휴대자병독기인형류행추세화분포특정，탐토기림상응용개치。방법：수궤수집무림상증상 HCV휴대자혈청양본공계90례，HCVRNA함량대우1×103 IU／ml ，기중남58례，녀32례。응용취합매련식반응（PCR）화반향반점잡교기술（RDB）진행HCV기인분형검측，병대수검자상관자료진행분석。결과：HCV휴대자 HCVRNA 평균농도위4．94±1．36 lg IU／ml。불동성별조지간 HCVRNA 함량무명현차이（P＞0．05）。소유표본HCV기인성공분형。단기인형감염위주요감염류형，점95．56％（86／90）。기중1b형감염솔위48．89％（44／90），2a형감염솔위42．22（38／90）。불동성별조 HCV휴대자 HCV기인형분포차이무통계학의의（P＞0．05）。결론：본지구 HCV휴대자감염주요류형위단기인형감염；HCV1b위주요기인형，기차위2a형；개전HCV기인형검측구유중요적림상의의。
Objective :To study the prevalence and distribution characteristics of hepatitis C virus (HCV) genotypes and to explore the clinic significance of HCV genotypes in HCV carriers .Methods :We conducted a cohort study with 90 participants including 58 male patients and 32 female patients .The HCV RNA levels of all sera sam‐ples were confirmed higher than 1 × 103 IU/ml .HCV genotyping was performed using type‐specific primers by means of polymerase chain reaction (PCR) and reverse dot blot (RDB) .The related data of all subjects were ana‐lyzed .GraphPad Prism 5 .0 software was used for statistical analysis .Results :The average concentration of HCV RNA in sera was 4 .94 ± 1 .36 log IU/ml .All 90 specimens were genotyped successfully .There were no significant difference between the different gender groups about the HCV RNA levels of all sera (P>0 .05) .HCV 1b was the most predominant genotype 48 .89% (44/90) ,followed by HCV 2a 42 .22% (38/90) .The single HCV genotype in‐fection was frequently detected among all patients .Its infection ratio was 95 .56% (86/90) .There was no significant difference between the different gender groups about the distribution of HCV genotypes(P>0 .05) .Conclusion :The single HCV genotype infection is primary infection style .HCV 1b is the most frequently detected genotype in pa‐tients infected with HCV ,following by HCV 2a .It is significant for clinical treatment to detect the genotypes of HCV .