东北林业大学学报
東北林業大學學報
동북임업대학학보
Journal of Northeast Forestry University
2015年
9期
107-110,120
,共5页
王有祥%徐兴军%邵淑丽%李怀永%吕建伟%王维禹%张伟伟%谢志刚%李旭艳
王有祥%徐興軍%邵淑麗%李懷永%呂建偉%王維禹%張偉偉%謝誌剛%李旭豔
왕유상%서흥군%소숙려%리부영%려건위%왕유우%장위위%사지강%리욱염
白腰朱顶雀%脱支酶%温度驯化%基础代谢率
白腰硃頂雀%脫支酶%溫度馴化%基礎代謝率
백요주정작%탈지매%온도순화%기출대사솔
Cardeu lis flammea%Debranching enzyme%Temperature domestication%Basal metabolic rate
为探讨温度对白腰朱顶雀( Carduelis flammea)生理生化指标的影响,以白腰朱顶雀为研究对象,将其分为低温组(5℃)、高温组(30℃)和对照组(23℃)进行驯化,驯化后测定其体质量、体脂质量分数(索氏抽提法)、器官的鲜质量和干质量(烘干恒质量法)、基础代谢率和脱支酶活性。结果表明:低温组体质量降低,在驯化0~2周内下降最为明显,第2周结束时与对照组差异极显著( p<0.01);高温组体质量增高,在驯化1~3周内涨幅较大,第1周结束时与对照组差异显著(p<0.05)。低温组基础代谢率显著高于高温组(p<0.05)。3组动物肌肉中脱支酶活性差异不显著( p>0.05);低温组肝脏中脱支酶活性与对照组差异极显著( p<0.01),高温组肝脏中脱支酶活性与对照组差异不显著(p>0.05),低温组肝脏中脱支酶活性与高温组差异极显著(p<0.01)。可见,温度是影响白腰朱顶雀产热特征改变的主要环境因子之一。低温条件下,白腰朱顶雀通过降低体质量、提高基础代谢率、增加摄入能来补偿能量消耗等一系列的生理生化反应,以适应外界环境温度的变化。
為探討溫度對白腰硃頂雀( Carduelis flammea)生理生化指標的影響,以白腰硃頂雀為研究對象,將其分為低溫組(5℃)、高溫組(30℃)和對照組(23℃)進行馴化,馴化後測定其體質量、體脂質量分數(索氏抽提法)、器官的鮮質量和榦質量(烘榦恆質量法)、基礎代謝率和脫支酶活性。結果錶明:低溫組體質量降低,在馴化0~2週內下降最為明顯,第2週結束時與對照組差異極顯著( p<0.01);高溫組體質量增高,在馴化1~3週內漲幅較大,第1週結束時與對照組差異顯著(p<0.05)。低溫組基礎代謝率顯著高于高溫組(p<0.05)。3組動物肌肉中脫支酶活性差異不顯著( p>0.05);低溫組肝髒中脫支酶活性與對照組差異極顯著( p<0.01),高溫組肝髒中脫支酶活性與對照組差異不顯著(p>0.05),低溫組肝髒中脫支酶活性與高溫組差異極顯著(p<0.01)。可見,溫度是影響白腰硃頂雀產熱特徵改變的主要環境因子之一。低溫條件下,白腰硃頂雀通過降低體質量、提高基礎代謝率、增加攝入能來補償能量消耗等一繫列的生理生化反應,以適應外界環境溫度的變化。
위탐토온도대백요주정작( Carduelis flammea)생리생화지표적영향,이백요주정작위연구대상,장기분위저온조(5℃)、고온조(30℃)화대조조(23℃)진행순화,순화후측정기체질량、체지질량분수(색씨추제법)、기관적선질량화간질량(홍간항질량법)、기출대사솔화탈지매활성。결과표명:저온조체질량강저,재순화0~2주내하강최위명현,제2주결속시여대조조차이겁현저( p<0.01);고온조체질량증고,재순화1~3주내창폭교대,제1주결속시여대조조차이현저(p<0.05)。저온조기출대사솔현저고우고온조(p<0.05)。3조동물기육중탈지매활성차이불현저( p>0.05);저온조간장중탈지매활성여대조조차이겁현저( p<0.01),고온조간장중탈지매활성여대조조차이불현저(p>0.05),저온조간장중탈지매활성여고온조차이겁현저(p<0.01)。가견,온도시영향백요주정작산열특정개변적주요배경인자지일。저온조건하,백요주정작통과강저체질량、제고기출대사솔、증가섭입능래보상능량소모등일계렬적생리생화반응,이괄응외계배경온도적변화。
In order to study the effect of the temperature on physiological and biochemical indexes of Carduelis flammea, the ex-perimental animals were randomly divided into low-temperature group ( 5℃) , high-temperature group ( 35℃) and the control group ( 23℃) , and after the domestication , the body weight , the body fat ( by soxhlet extraction method ) , the fresh weight, dry weight (drying constant weight method ) of organs, basal metabolic rate and debranching enzyme activity were determined.The low-temperature group weight reduced , and during the domestication within 0-2 weeks, it was de-clined significantly with extremely significant difference at the end of second week compared with control group ( p<0.01) . The high-temperature group weight increased ,during the domestication within 1-3 weeks, it was increased significantly with extremely significant difference at the end of first week compared with control group ( p<0.05) .Low-temperature group basal metabolic rate was significantly higher than that in high-temperature group ( p<0.05) .Three groups of animal muscle debranching enzyme activity was not significantly different ( p>0.05) .Low-temperature group debranching enzyme activity in the liver was significantly different compared with the control group ( p<0.01) , but for the difference between high-tem-perature group debranching enzyme activity in the liver compared with the control group , there was no significant difference (p>0.05).The difference between the low-temperature group debranching enzyme activity in the liver that in high-tempera-ture group was significant (p<0.01).Therefore, temperature is one of the major environmental factors affecting C.flammea thermogenesis .Under low temperature , C.flammea can reduce body weight , improve the metabolic rate , and increase en-ergy intake to compensate the energy consumption to adapt the temperature changes of external environment .