中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2015年
10期
585-589
,共5页
罗亮%舒美兰%李舒眉%蔡扬
囉亮%舒美蘭%李舒眉%蔡颺
라량%서미란%리서미%채양
扁平苔藓,口腔%免疫%可溶性程序性死亡受体1%可溶性程序性死亡配体1
扁平苔蘚,口腔%免疫%可溶性程序性死亡受體1%可溶性程序性死亡配體1
편평태선,구강%면역%가용성정서성사망수체1%가용성정서성사망배체1
Lichen planus,oral%Immunity%Soluble programmed death-1(sPD-1)%Soluble programmed death ligand 1
目的 了解口腔扁平苔藓(oral lichen planus,OLP)患者血清中可溶性程序性死亡受体1(soluble programmed death-1,sPD-1)、可溶性程序性死亡配体1(soluble programmed death ligand 1,sPD-L1)蛋白表达与患者免疫功能状态的相关性,探讨sPD-1/sPD-L1在OLP免疫发病机制中的作用及意义.方法 选择2012年9月至2013年9月贵阳医学院附属医院口腔内科黏膜病专科门诊的OLP患者36例(网纹型20例、糜烂型16例)(OLP组)及18名健康对照者(对照组),采用流式细胞术分析OLP患者外周血淋巴细胞亚群(CD3+、CD4+、CD8+、CD19+、CD16++56+)状况,散射比浊法检测患者体液免疫指标(IgG、IgA、IgM、C3、C4),并与实验室正常值进行比较;酶联免疫吸附剂测定法检测两组受试者血清中sPD-1、sPD-L1蛋白的表达情况,并分析sPD-1、sPD-L1表达水平与OLP患者免疫功能状况及临床特征的相关性.结果 与实验室正常值相比,OLP患者细胞免疫中CD3+、CD4+、CD8+、CD16++56+表达水平均显著低于正常值,CD19+水平显著高于正常值(P＜0.05);体液免疫中C3、C4表达水平显著低于正常值,IgM水平显著高于正常值(P＜0.05).OLP患者血清中sPD-1及sPD-L1蛋白的表达量[分别为26.10(8.81,40.00)及29.53(21.47,36.76) ng/L]均显著高于对照组[分别为17.65(0.00,26.10)及22.79(1.19,28.29) ng/L](P＜0.05);但sPD-1、sPD-L1表达水平与OLP临床特征均无相关性(P＞0.05);sPD-1表达量与OLP患者CD4+(r=-0.378,P=0.007)及CD16++56+(r=-0.365,P=0.009)存在负相关关系,与CD19+存在正相关关系(r=0.482,P=0.000);sPD-L1表达量与CD4+存在负相关关系(r=-0.286,P=0.044),与IgG存在正相关关系(r=0.365,P=0.029).结论 OLP患者以细胞免疫功能低下为主,同时伴有体液免疫功能紊乱;sPD-1、sPD-L1蛋白的异常高表达在一定程度上通过影响PD-1/PD-L1信号通路参与OLP免疫机制的调节,在OLP的免疫发病机制中起一定作用.
目的 瞭解口腔扁平苔蘚(oral lichen planus,OLP)患者血清中可溶性程序性死亡受體1(soluble programmed death-1,sPD-1)、可溶性程序性死亡配體1(soluble programmed death ligand 1,sPD-L1)蛋白錶達與患者免疫功能狀態的相關性,探討sPD-1/sPD-L1在OLP免疫髮病機製中的作用及意義.方法 選擇2012年9月至2013年9月貴暘醫學院附屬醫院口腔內科黏膜病專科門診的OLP患者36例(網紋型20例、糜爛型16例)(OLP組)及18名健康對照者(對照組),採用流式細胞術分析OLP患者外週血淋巴細胞亞群(CD3+、CD4+、CD8+、CD19+、CD16++56+)狀況,散射比濁法檢測患者體液免疫指標(IgG、IgA、IgM、C3、C4),併與實驗室正常值進行比較;酶聯免疫吸附劑測定法檢測兩組受試者血清中sPD-1、sPD-L1蛋白的錶達情況,併分析sPD-1、sPD-L1錶達水平與OLP患者免疫功能狀況及臨床特徵的相關性.結果 與實驗室正常值相比,OLP患者細胞免疫中CD3+、CD4+、CD8+、CD16++56+錶達水平均顯著低于正常值,CD19+水平顯著高于正常值(P＜0.05);體液免疫中C3、C4錶達水平顯著低于正常值,IgM水平顯著高于正常值(P＜0.05).OLP患者血清中sPD-1及sPD-L1蛋白的錶達量[分彆為26.10(8.81,40.00)及29.53(21.47,36.76) ng/L]均顯著高于對照組[分彆為17.65(0.00,26.10)及22.79(1.19,28.29) ng/L](P＜0.05);但sPD-1、sPD-L1錶達水平與OLP臨床特徵均無相關性(P＞0.05);sPD-1錶達量與OLP患者CD4+(r=-0.378,P=0.007)及CD16++56+(r=-0.365,P=0.009)存在負相關關繫,與CD19+存在正相關關繫(r=0.482,P=0.000);sPD-L1錶達量與CD4+存在負相關關繫(r=-0.286,P=0.044),與IgG存在正相關關繫(r=0.365,P=0.029).結論 OLP患者以細胞免疫功能低下為主,同時伴有體液免疫功能紊亂;sPD-1、sPD-L1蛋白的異常高錶達在一定程度上通過影響PD-1/PD-L1信號通路參與OLP免疫機製的調節,在OLP的免疫髮病機製中起一定作用.
목적 료해구강편평태선(oral lichen planus,OLP)환자혈청중가용성정서성사망수체1(soluble programmed death-1,sPD-1)、가용성정서성사망배체1(soluble programmed death ligand 1,sPD-L1)단백표체여환자면역공능상태적상관성,탐토sPD-1/sPD-L1재OLP면역발병궤제중적작용급의의.방법 선택2012년9월지2013년9월귀양의학원부속의원구강내과점막병전과문진적OLP환자36례(망문형20례、미란형16례)(OLP조)급18명건강대조자(대조조),채용류식세포술분석OLP환자외주혈림파세포아군(CD3+、CD4+、CD8+、CD19+、CD16++56+)상황,산사비탁법검측환자체액면역지표(IgG、IgA、IgM、C3、C4),병여실험실정상치진행비교;매련면역흡부제측정법검측량조수시자혈청중sPD-1、sPD-L1단백적표체정황,병분석sPD-1、sPD-L1표체수평여OLP환자면역공능상황급림상특정적상관성.결과 여실험실정상치상비,OLP환자세포면역중CD3+、CD4+、CD8+、CD16++56+표체수평균현저저우정상치,CD19+수평현저고우정상치(P＜0.05);체액면역중C3、C4표체수평현저저우정상치,IgM수평현저고우정상치(P＜0.05).OLP환자혈청중sPD-1급sPD-L1단백적표체량[분별위26.10(8.81,40.00)급29.53(21.47,36.76) ng/L]균현저고우대조조[분별위17.65(0.00,26.10)급22.79(1.19,28.29) ng/L](P＜0.05);단sPD-1、sPD-L1표체수평여OLP림상특정균무상관성(P＞0.05);sPD-1표체량여OLP환자CD4+(r=-0.378,P=0.007)급CD16++56+(r=-0.365,P=0.009)존재부상관관계,여CD19+존재정상관관계(r=0.482,P=0.000);sPD-L1표체량여CD4+존재부상관관계(r=-0.286,P=0.044),여IgG존재정상관관계(r=0.365,P=0.029).결론 OLP환자이세포면역공능저하위주,동시반유체액면역공능문란;sPD-1、sPD-L1단백적이상고표체재일정정도상통과영향PD-1/PD-L1신호통로삼여OLP면역궤제적조절,재OLP적면역발병궤제중기일정작용.
Objective To investigate the possible role and significance of soluble programmed death-1 (sPD-1)/soluble programmed death ligand 1 (sPD-L1) in the pathogenesis of oral lichen planus(OLP).Methods Thirty-six patients with OLP(20 cases of reticular OLP and 16 cases of erosive OLP) were enrolled in this study,and 18 healthy people served as controls.Lymphocyte subsets(CD3+,CD4+,CD8+,CD19+,CD16++56+) were examined by flow cytometric analysis and humoral immunity indexes(IgG,IgA,IgM,C3,C4) tested by nephelometry immunoassay.The levels of sPD-1 and sPD-L1 proteins in serum of patients with OLP were determined by enzyme-linked immunosorbent assay.The correlations between the level of sPD-1,sPD-L1 proteins and the immune status and clinical characteristics of patients with OLP were analyzed by SPSS 19.0.Results CD3+,CD4+,CD8+,CD16++56+ in patients with OLP were decreased compared with the normal value,while CD19+ in patients with OLP was increased compared with the normal value(P＜0.05).C3 and C4 in patients with OLP were decreased compared with the normal value,but IgM in patients with OLP was increased(P＜0.05).The levels of sPD-1 and sPD-L1 proteins in patients with OLP were significantly higher than that in control group[26.10(8.81,40.00) ng/L vs 17.65(0.00,26.10) ng/L,29.53 (21.47,36.76) ng/L vs 22.79(1.19,28.29) ng/L](P＜0.05),but the expression of sPD-1 and sPD-L1 was not related with clinical characteristics of OLP.There were negative correlations between the levels of sPD-1 protein and CD4+T cells or CD 16++56+ cells(r1=-0.378,P1=0.007;r2=-0.365,P2=0.009),while there was a positive correlation between the levels of sPD-1 and CD19+B cells(r=0.482,P=0.000).There was a negative correlation between sPD-L1 expression level and CD4 + and a positive correlation between sPD-L1 expression level and IgG(r1=-0.286,P1=0.044;r2=0.365,P2=0.029).Conclusions In patients with OLP,the cellular immune function is low with humoral immunity function disorder.PD-1/PD-L1 signaling pathway,which might be influenced by the involvement of sPD-1 and sPD-L1 proteins in a certain extent,may play an important role in the immune pathogenesis of OLP.
