中华儿科杂志
中華兒科雜誌
중화인과잡지
Chinese Journal of Pediatrics
2015年
10期
765-770
,共6页
马全萍%刘雅毳%刘靓雯%姚明晓%苑广盈
馬全萍%劉雅毳%劉靚雯%姚明曉%苑廣盈
마전평%류아취%류정문%요명효%원엄영
志贺菌属%抗药性%整合子类
誌賀菌屬%抗藥性%整閤子類
지하균속%항약성%정합자류
Shigella%Drug resistance%Integrons
目的 探讨福氏志贺菌多重耐药性与其整合子携带的耐药基因盒的关系.方法 收集2009年5月至2012年4月济南市部分医院就诊的6个月～14岁腹泻患儿粪便分离的福氏志贺菌79株,Kirby-Bauer法(K-B纸片扩散法)测定福氏志贺菌的耐药性,PCR扩增1、2、3类整合子酶基因,对阳性菌株可变区进行酶切和序列分析.结果 分离的79株福氏志贺菌对氨苄西林、氯霉素、四环素、链霉素的耐药率均为91％(72/79),磺胺甲基异嗯唑-甲氧苄啶的耐药率为70％ (55/79),对头孢噻肟、头孢他啶、环丙沙星和左旋氧氟沙星的耐药率分别为30％(24/79)、23％ (18/79)、33％(26/79)、32％(25/79),对头孢西丁、头孢哌酮-舒巴坦、亚胺培南100％敏感.常见的耐药模式为氨苄西林-四环素-氯霉素-链霉素,占91％(72/79).79株福氏志贺菌中,intI1阳性72株、intI2阳性68株,阳性率分别为91％(72/79)、86％(68/79),intI3均阴性.非典型1类整合子阳性菌株对氨苄西林、链霉素、四环素、氯霉素耐药明显高于非典型1类整合子阴性菌株(x2=35.96,P＜0.01).测序结果经BLAST比对:1类整合子可变区检出9株dfrV、2株dfrA17-aadA5、70株blaOXA-30-aadA1耐药基因盒,2株可变区未检出耐药基因.2类整合子可变区全部检出dfrA1-sat1-aadA1耐药基因盒.结论 济南地区福氏志贺菌存在的多重耐药与携带的整合子密切相关.
目的 探討福氏誌賀菌多重耐藥性與其整閤子攜帶的耐藥基因盒的關繫.方法 收集2009年5月至2012年4月濟南市部分醫院就診的6箇月～14歲腹瀉患兒糞便分離的福氏誌賀菌79株,Kirby-Bauer法(K-B紙片擴散法)測定福氏誌賀菌的耐藥性,PCR擴增1、2、3類整閤子酶基因,對暘性菌株可變區進行酶切和序列分析.結果 分離的79株福氏誌賀菌對氨芐西林、氯黴素、四環素、鏈黴素的耐藥率均為91％(72/79),磺胺甲基異嗯唑-甲氧芐啶的耐藥率為70％ (55/79),對頭孢噻肟、頭孢他啶、環丙沙星和左鏇氧氟沙星的耐藥率分彆為30％(24/79)、23％ (18/79)、33％(26/79)、32％(25/79),對頭孢西丁、頭孢哌酮-舒巴坦、亞胺培南100％敏感.常見的耐藥模式為氨芐西林-四環素-氯黴素-鏈黴素,佔91％(72/79).79株福氏誌賀菌中,intI1暘性72株、intI2暘性68株,暘性率分彆為91％(72/79)、86％(68/79),intI3均陰性.非典型1類整閤子暘性菌株對氨芐西林、鏈黴素、四環素、氯黴素耐藥明顯高于非典型1類整閤子陰性菌株(x2=35.96,P＜0.01).測序結果經BLAST比對:1類整閤子可變區檢齣9株dfrV、2株dfrA17-aadA5、70株blaOXA-30-aadA1耐藥基因盒,2株可變區未檢齣耐藥基因.2類整閤子可變區全部檢齣dfrA1-sat1-aadA1耐藥基因盒.結論 濟南地區福氏誌賀菌存在的多重耐藥與攜帶的整閤子密切相關.
목적 탐토복씨지하균다중내약성여기정합자휴대적내약기인합적관계.방법 수집2009년5월지2012년4월제남시부분의원취진적6개월～14세복사환인분편분리적복씨지하균79주,Kirby-Bauer법(K-B지편확산법)측정복씨지하균적내약성,PCR확증1、2、3류정합자매기인,대양성균주가변구진행매절화서렬분석.결과 분리적79주복씨지하균대안변서림、록매소、사배소、련매소적내약솔균위91％(72/79),광알갑기이은서-갑양변정적내약솔위70％ (55/79),대두포새우、두포타정、배병사성화좌선양불사성적내약솔분별위30％(24/79)、23％ (18/79)、33％(26/79)、32％(25/79),대두포서정、두포고동-서파탄、아알배남100％민감.상견적내약모식위안변서림-사배소-록매소-련매소,점91％(72/79).79주복씨지하균중,intI1양성72주、intI2양성68주,양성솔분별위91％(72/79)、86％(68/79),intI3균음성.비전형1류정합자양성균주대안변서림、련매소、사배소、록매소내약명현고우비전형1류정합자음성균주(x2=35.96,P＜0.01).측서결과경BLAST비대:1류정합자가변구검출9주dfrV、2주dfrA17-aadA5、70주blaOXA-30-aadA1내약기인합,2주가변구미검출내약기인.2류정합자가변구전부검출dfrA1-sat1-aadA1내약기인합.결론 제남지구복씨지하균존재적다중내약여휴대적정합자밀절상관.
Objective To investigate the correlation between Shigella flexneri multi-drug resistance and drug resistance gene cassette of integrons.Method All 79 strains of Shigella flexneri were isolated from the feces of children ranged in age from 6 months to 14 years in some hospitals of Jinan,between May 2009 and April 2012.The resistance was detected by Kirby Bauer agar diffusion method,1,2 and 3 integron gene was amplified by PCR,the variable region of positive strains treated with enzyme digestion and determined by Series Analysis.Result Among 79 Shigella flexneri strains,the resistance rate was 91％ (72/79) to ampicillin,chloramphenicol,tetracycline,streptomycin,70％ (55/79) to sulfamethoxazole/trimethoprim,30％ (24/79),23％ (18/79),33％ (26/79) and 32％ (25/79) to cefotaxime,ceftazidime,ciprofloxacin and levofloxacin.All 79 strains were susceptible to cefoxitin,imipenem,cefoperazone/sulbactam.The common drug resistance pattern is ampicillin tetracycline-chloramphenicol-streptomycin,accounted for 91％ (72/79);91％ (72/79) strains carried integrons of class 1,86％ (68/79) strains carried integrons of class 2,No intI3 was detected.The resistance to ampicillin,streptomycin,tetracycline,chloramphenicol of atypical class 1 integron positive strains was significantly higher than the negative strains(x2 =35.96,P ＜ 0.01).The sequencing results:dfrV was detected in class 1 integron variable regions of 9 strains,dfrA17-aadA5 in 2 strains,blaOXA-30-aadA1 in 70 strains,2 strains were not detected resistance gene cassette,all resistance gene cassettes were dfrA1-sat1-aadA1 in class 2 integron variable regions.Conclusion The mutidrug resistance of Shigella flexneri in Jinan was closely associated with integrons.
