齐齐哈尔医学院学报
齊齊哈爾醫學院學報
제제합이의학원학보
Journal of Qiqihar University of Medicine
2015年
27期
4060-4063
,共4页
李云龙%王伟录%梁东彦%李巧星
李雲龍%王偉錄%樑東彥%李巧星
리운룡%왕위록%량동언%리교성
膀胱癌%吡柔比星%细胞穿膜肽%单克隆抗体
膀胱癌%吡柔比星%細胞穿膜肽%單剋隆抗體
방광암%필유비성%세포천막태%단극륭항체
Bladder cancer%THP%Cell-penetrating peptides%Monoclonal antibodies
目的 探讨CPPs-BDI-1-载吡柔比星白蛋白纳米微球三联体杀伤人膀胱癌细胞研究,为后续实验构建靶向膀胱癌的新型药物传送系统及治疗奠定基础. 方法 采用异型双功能连接剂SPDP耦联穿膜肽增强型绿色荧光蛋白(TAT-EGFP)、抗人膀胱癌单克隆抗体(BDI-1)、载吡柔比星白蛋白纳米微球( THP-NS) ,构建载吡柔比星白蛋白纳米微球三联体( CPPs-BDI-1-THP-NS). 取CPPs-BDI-1-THP-NS三联体实验组和吡柔比星对照组,通过MTT法检测CPPs-BDI-1-THP-NS三联体对EJ人膀胱癌细胞的杀伤效果;Annexin V-FITC/PI法检测膀胱癌细胞的凋亡情况. 结果 CPPs-BDI-1-载吡柔比星白蛋白纳米微球三联体实验组杀伤人膀胱癌细胞作用明显强于对照组.结论 CPPs-BDI-1-载吡柔比星白蛋白纳米微球三联对人膀胱癌细胞有明显的杀伤作用.
目的 探討CPPs-BDI-1-載吡柔比星白蛋白納米微毬三聯體殺傷人膀胱癌細胞研究,為後續實驗構建靶嚮膀胱癌的新型藥物傳送繫統及治療奠定基礎. 方法 採用異型雙功能連接劑SPDP耦聯穿膜肽增彊型綠色熒光蛋白(TAT-EGFP)、抗人膀胱癌單剋隆抗體(BDI-1)、載吡柔比星白蛋白納米微毬( THP-NS) ,構建載吡柔比星白蛋白納米微毬三聯體( CPPs-BDI-1-THP-NS). 取CPPs-BDI-1-THP-NS三聯體實驗組和吡柔比星對照組,通過MTT法檢測CPPs-BDI-1-THP-NS三聯體對EJ人膀胱癌細胞的殺傷效果;Annexin V-FITC/PI法檢測膀胱癌細胞的凋亡情況. 結果 CPPs-BDI-1-載吡柔比星白蛋白納米微毬三聯體實驗組殺傷人膀胱癌細胞作用明顯彊于對照組.結論 CPPs-BDI-1-載吡柔比星白蛋白納米微毬三聯對人膀胱癌細胞有明顯的殺傷作用.
목적 탐토CPPs-BDI-1-재필유비성백단백납미미구삼련체살상인방광암세포연구,위후속실험구건파향방광암적신형약물전송계통급치료전정기출. 방법 채용이형쌍공능련접제SPDP우련천막태증강형록색형광단백(TAT-EGFP)、항인방광암단극륭항체(BDI-1)、재필유비성백단백납미미구( THP-NS) ,구건재필유비성백단백납미미구삼련체( CPPs-BDI-1-THP-NS). 취CPPs-BDI-1-THP-NS삼련체실험조화필유비성대조조,통과MTT법검측CPPs-BDI-1-THP-NS삼련체대EJ인방광암세포적살상효과;Annexin V-FITC/PI법검측방광암세포적조망정황. 결과 CPPs-BDI-1-재필유비성백단백납미미구삼련체실험조살상인방광암세포작용명현강우대조조.결론 CPPs-BDI-1-재필유비성백단백납미미구삼련대인방광암세포유명현적살상작용.
Objective To explore the experimental research of CPPs -BDI-1-THP-Loaded albumin nanoparticle triad killing EJ human bladder cancer cells , in other to build the new drug delivery system of targeting for follow -up experiments and treatment of bladder cancer .Methods SPDP was use to decoupling league to build nanometer microspheres triplet CPPs -BDI-THP-NS with the preparation of TAT -EGFP, BDI-1-NS and THP-NS.Take CPPs -BDI-1-THP-NS triplets as experimental group and THP as control group to detect the kill effect on EJ human bladder cancer cells by MTT method and detecting bladder cancer cells apoptosis by Annexin V-FITC/PI method.Results The inhibition effect of CPPs -BDI-1-THP-NS triplet killing EJ human bladder cancer cells was obviously stronger than the control group .Conclusions CPPs-BDI-1-THP-NS triplets has an obvious killing effect on human bladder cancer cells .