高等学校化学学报
高等學校化學學報
고등학교화학학보
Chemical Journal of Chinese Universities
2015年
10期
1906-1911
,共6页
欧阳巧凤%郑兰兰%曹红%张馨%李春%沈姣%陈礼婷%刘进
歐暘巧鳳%鄭蘭蘭%曹紅%張馨%李春%瀋姣%陳禮婷%劉進
구양교봉%정란란%조홍%장형%리춘%침교%진례정%류진
疏水性离子液体%N-甲基-N-丁基吡咯烷双三氟甲烷磺酰亚胺盐(MBPyTf2 N)%光谱特征%荧光检测法%生物催化体系
疏水性離子液體%N-甲基-N-丁基吡咯烷雙三氟甲烷磺酰亞胺鹽(MBPyTf2 N)%光譜特徵%熒光檢測法%生物催化體繫
소수성리자액체%N-갑기-N-정기필각완쌍삼불갑완광선아알염(MBPyTf2 N)%광보특정%형광검측법%생물최화체계
Hydrophobic ionic liquid%N-Methyl-N-butyl pyrrolidinium bis[(trifluoromethyl) sulfonyl] imide ([MBPy]Tf2 N)%Spectral characterization%Fluorescence detection method%Biological catalysis system
为满足疏水性离子液体 N-甲基-N-丁基吡咯烷双三氟甲烷磺酰亚胺盐([MBPy]Tf2 N)在甘草酸生物催化体系中分析检测的需要,研究了[MBPy]Tf2 N 的光谱特征,并考察了溶剂、醇-水混合溶剂和 pH 值对[MB-Py]Tf2 N 荧光特性的影响,建立了生物催化体系中[MBPy]Tf2 N 的荧光光谱检测方法.研究结果表明,[MB-Py]Tf2 N 在186 nm 处有1个最大吸收峰,常规紫外检测法不适于对其进行检测；[MBPy]Tf2 N 荧光光谱最大激发波长为228 nm,发射波长为340 nm,且其耐光褪色性良好,在水溶液中荧光量子产率为0.067,在醇-水混合溶剂中其荧光强度有增大趋势,在碱性介质中其荧光强度受影响较大；荧光光谱法检测[MBPy]Tf2 N 的检出限(S/ N =3)为1.00μg/ mL,标准曲线在5.00~1.25×103μg/ mL 浓度范围内线性关系良好( R2=0.9999),生物反应液中加标回收率为97.4%~107.4%(相对标准偏差 RSD=4.6%).
為滿足疏水性離子液體 N-甲基-N-丁基吡咯烷雙三氟甲烷磺酰亞胺鹽([MBPy]Tf2 N)在甘草痠生物催化體繫中分析檢測的需要,研究瞭[MBPy]Tf2 N 的光譜特徵,併攷察瞭溶劑、醇-水混閤溶劑和 pH 值對[MB-Py]Tf2 N 熒光特性的影響,建立瞭生物催化體繫中[MBPy]Tf2 N 的熒光光譜檢測方法.研究結果錶明,[MB-Py]Tf2 N 在186 nm 處有1箇最大吸收峰,常規紫外檢測法不適于對其進行檢測；[MBPy]Tf2 N 熒光光譜最大激髮波長為228 nm,髮射波長為340 nm,且其耐光褪色性良好,在水溶液中熒光量子產率為0.067,在醇-水混閤溶劑中其熒光彊度有增大趨勢,在堿性介質中其熒光彊度受影響較大；熒光光譜法檢測[MBPy]Tf2 N 的檢齣限(S/ N =3)為1.00μg/ mL,標準麯線在5.00~1.25×103μg/ mL 濃度範圍內線性關繫良好( R2=0.9999),生物反應液中加標迴收率為97.4%~107.4%(相對標準偏差 RSD=4.6%).
위만족소수성리자액체 N-갑기-N-정기필각완쌍삼불갑완광선아알염([MBPy]Tf2 N)재감초산생물최화체계중분석검측적수요,연구료[MBPy]Tf2 N 적광보특정,병고찰료용제、순-수혼합용제화 pH 치대[MB-Py]Tf2 N 형광특성적영향,건립료생물최화체계중[MBPy]Tf2 N 적형광광보검측방법.연구결과표명,[MB-Py]Tf2 N 재186 nm 처유1개최대흡수봉,상규자외검측법불괄우대기진행검측；[MBPy]Tf2 N 형광광보최대격발파장위228 nm,발사파장위340 nm,차기내광퇴색성량호,재수용액중형광양자산솔위0.067,재순-수혼합용제중기형광강도유증대추세,재감성개질중기형광강도수영향교대；형광광보법검측[MBPy]Tf2 N 적검출한(S/ N =3)위1.00μg/ mL,표준곡선재5.00~1.25×103μg/ mL 농도범위내선성관계량호( R2=0.9999),생물반응액중가표회수솔위97.4%~107.4%(상대표준편차 RSD=4.6%).
The detection method of hydrophobic ionic liquid N-methyl-N-butyl pyrrolidinium bis[(trifluoro-methyl)sulfonyl]imide([MBPy]Tf2 N) was established by means of fluorescence spectrum and applied for the analysis of [MBPy] Tf2 N from glycyrrhizin biological catalysis system containing ionic liquid. The effects of solvent, ethanol-water mixture solvent and pH on fluorescence spectrum were explored. Spectral features of [MBPy]Tf2 N, the maximum absorption wavelength at 186 nm, were apparently cannot detect [MBPy]Tf2 N by common UV method. The maximum excitation wavelength of [MBPy]Tf2 N is at 228 nm and the maximum emission wavelength of it is at 340 nm, which can well againsted photobleaching and the fluorescence quantum yield was 0. 067 in water. The fluorescence intensity increased in ethanol-water mixture solvent and changed much in alkaline medium. The fluorescence detection method for [MBPy]Tf2 N had a wide linear range over [MBPy]Tf2 N concentration from 5. 00 to 1. 25×103μg / mL with a detection limit of 1. 00 μg / mL(S / N = 3). The spiked recoveries were from 97. 4% to 107. 4% (RSD=4. 6% ) in glycyrrhizin biological catalysis system.
