高等学校化学学报
高等學校化學學報
고등학교화학학보
Chemical Journal of Chinese Universities
2015年
10期
1900-1905
,共6页
杨津%刘卓%王晓慧%隋会敏%毛竹%赵冰
楊津%劉卓%王曉慧%隋會敏%毛竹%趙冰
양진%류탁%왕효혜%수회민%모죽%조빙
2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐(WST-8)%表面增强拉曼光谱%细胞活性检测
2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑單鈉鹽(WST-8)%錶麵增彊拉曼光譜%細胞活性檢測
2-(2-갑양기-4-초분기)-3-(4-초분기)-5-(2,4-이광기분)-2H-사서단납염(WST-8)%표면증강랍만광보%세포활성검측
2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monoso-dium salt(WST-8)%Surface-enhanced Raman scattering(SERS)%Cell viability detection
采用常规的用于测定活细胞个数的试剂2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐(WST-8四唑盐)建立了一种快速测定细胞活性的表面增强拉曼散射光谱(SERS)新方法.考察了pH 值及还原剂烟酰胺腺嘌噙二核苷酸(NADH)用量对 WST-8甲瓒信号的影响.结果表明,与传统的 WST-8比色法相比, SERS 法具有更高的检测灵敏度和选择性,对 WST-8甲瓒的检测限可达到10μmol/ L.
採用常規的用于測定活細胞箇數的試劑2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑單鈉鹽(WST-8四唑鹽)建立瞭一種快速測定細胞活性的錶麵增彊拉曼散射光譜(SERS)新方法.攷察瞭pH 值及還原劑煙酰胺腺嘌噙二覈苷痠(NADH)用量對 WST-8甲瓚信號的影響.結果錶明,與傳統的 WST-8比色法相比, SERS 法具有更高的檢測靈敏度和選擇性,對 WST-8甲瓚的檢測限可達到10μmol/ L.
채용상규적용우측정활세포개수적시제2-(2-갑양기-4-초분기)-3-(4-초분기)-5-(2,4-이광기분)-2H-사서단납염(WST-8사서염)건립료일충쾌속측정세포활성적표면증강랍만산사광보(SERS)신방법.고찰료pH 치급환원제연선알선표금이핵감산(NADH)용량대 WST-8갑찬신호적영향.결과표명,여전통적 WST-8비색법상비, SERS 법구유경고적검측령민도화선택성,대 WST-8갑찬적검측한가체도10μmol/ L.
Surface-enhanced Raman scattering(SERS) technique was used to develop and optimize a novel and quantitative 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt(WST-8) assay for living cell viability. The influences of pH and dosage of reductant nicoti-namide adenine dinucleotide(NADH) on WST-8 formazan signal were examined. SERS signals of formazan with high resolution and great diversity between WST-8 and formazan were observed in neutral conditions. Meanwhile, the signals of formazan(560, 1351, 1598, 1616 cm-1 ) could be found clearly when the concen-tration of NADH was four times that of WST-8. Compared with the traditional method, this assay appears higher sensitivity and selectivity for WST-8 formazan. Moreover, the detection limit of formazan is as low as 10μmol/ L.
