CAJ | 학술논문

目的：探讨氧化苦参碱对人子宫内膜癌细胞HEC‐1‐B增殖、侵袭转移的作用及其机制。方法：MTT法检测细胞增殖抑制率；Transw ell 小室法观察细胞侵袭转移的改变；Western blot及Real‐time PCR法检测p38、p‐p38、MMP‐2和MMP‐9表达水平的变化。结果：一定浓度氧化苦参碱可显著抑制子宫内膜癌细胞HEC‐1‐B的增殖、侵袭转移，并呈时间和剂量依赖性。氧化苦参碱可显著降低H EC‐1‐B细胞中p38及MMPs的表达水平。联合应用SB203580能够有效增强氧化苦参碱的抗肿瘤细胞侵袭转移能力。结论：氧化苦参碱可以抑制子宫内膜癌细胞的增殖、侵袭转移，并下调p38磷酸化以及MMP2、MMP9的表达。
목적：탐토양화고삼감대인자궁내막암세포HEC‐1‐B증식、침습전이적작용급기궤제。방법：MTT법검측세포증식억제솔；Transw ell 소실법관찰세포침습전이적개변；Western blot급Real‐time PCR법검측p38、p‐p38、MMP‐2화MMP‐9표체수평적변화。결과：일정농도양화고삼감가현저억제자궁내막암세포HEC‐1‐B적증식、침습전이，병정시간화제량의뢰성。양화고삼감가현저강저H EC‐1‐B세포중p38급MMPs적표체수평。연합응용SB203580능구유효증강양화고삼감적항종류세포침습전이능력。결론：양화고삼감가이억제자궁내막암세포적증식、침습전이，병하조p38린산화이급MMP2、MMP9적표체。
Objective:To investigate the effects and mechanisms of oxymatrine on invasion and metastasis of Endometrial carcinoma HEC‐1‐B cells .Methods :HEC‐1‐B cells were cultured in vitro and treated by 0 ,0 .5 ,1 .0 and 1 .5 mg/ml oxymatrine .Then cell growth curves assay and cell invasion assay using Transwell chamber was used to evaluate the effects of oxymatrine on invasion of HEC‐1‐B cells .The expression of p38 ,p‐p38 ,matrix metal‐loproteinases‐2(MMP‐2) and MMP‐9 were analysed by Western blot and Real‐time PCR .Results :Compared with control group ,oxymatrine (0 .5 ,1 .0 and 1 .5 mg/ml) treated groups significantly inhibited the proliferation and inva‐sive ability of HEC‐1‐B cells in a concentration‐and time‐dependent manner .In addition ,oxymatrine reduced the phosphorylation level of p38 protein and the mRNA levels of MMP‐2 and MMP‐9 .Moreover ,a p38 inhibitor‐SB203580 and oxymatrine excert synergistic inhibition effects on the expression of MMP‐2 and MMP‐9 in HEC‐1‐B cells .Conclusion:These findings suggest that a potential mechanism by which oxymatrine has an anti‐metastatic effects on endometrial cancer cells via directly down‐regulating the p38 signaling pathway .