CAJ | 학술논문

采用溶剂热法,通过有机单体合成了一种亚胺键连接的共价有机框架材料(COF-LZU1)；在常温常压条件下,通过后合成的方法将贵金属钯(Ⅱ)引入到 COF 材料中,合成了复合材料 Pd/ COF-LZU1,该材料具有优良的催化性能.利用 Pd/ COF-LZU1多孔复合材料将 C-反应蛋白(CRP)抗体(anti-CRP)固定在玻碳电极表面,构建了一种非标记型 CRP 免疫传感器.当抗体与抗原发生免疫反应时,形成的免疫复合物会阻碍电化学探针[Fe(CN)6]4-/3-的电子传递,降低其响应电流,从而实现 CRP 的快速检测.采用交流阻抗和差示脉冲伏安法(DPV)考察了免疫传感器的电化学特性,同时考察了测试底液的 pH 值、抗原培育时间和抗体固定浓度等实验条件对传感器性能的影响.在最优的实验条件下,采用 DPV 法对 CRP 进行检测的线性范围为5~180 ng/ mL,检出限为1.66 ng/ mL,线性相关系数为0.992.
채용용제열법,통과유궤단체합성료일충아알건련접적공개유궤광가재료(COF-LZU1)；재상온상압조건하,통과후합성적방법장귀금속파(Ⅱ)인입도 COF 재료중,합성료복합재료 Pd/ COF-LZU1,해재료구유우량적최화성능.이용 Pd/ COF-LZU1다공복합재료장 C-반응단백(CRP)항체(anti-CRP)고정재파탄전겁표면,구건료일충비표기형 CRP 면역전감기.당항체여항원발생면역반응시,형성적면역복합물회조애전화학탐침[Fe(CN)6]4-/3-적전자전체,강저기향응전류,종이실현 CRP 적쾌속검측.채용교류조항화차시맥충복안법(DPV)고찰료면역전감기적전화학특성,동시고찰료측시저액적 pH 치、항원배육시간화항체고정농도등실험조건대전감기성능적영향.재최우적실험조건하,채용 DPV 법대 CRP 진행검측적선성범위위5~180 ng/ mL,검출한위1.66 ng/ mL,선성상관계수위0.992.
A new imine-linked covalent organic framework ( COF) material was synthesized using organic monomer via solvothermal methods. Pd(Ⅱ)-coordinated COF, Pd / COF-LZU1, was accordingly synthesized through simple post-treatment at ambient conditions, which showed excellent catalytic activity. Then, a label-free C-reactive protein(CRP) immunosensor was constructed using the Pd / COF-LZUI porous composite mate-rial to immobilize anti-CRP on the surface of GCE. When anti-CRP binds with CRP antigen, the formed im-muno complex obstruct the electric signal of Fe(CN) 6 4- / 3-, which can be used to realize label-free detection of CRP. Electrochemical impedence spectroscopy(EIS) and differential pulse voltammetry(DPV) were employed to characterize the immunosensor. The experimental conditions such as scanning rate, pH value of testing solu-tion, the concentration of anti-CRP and incubation time were also optimized. Under the optimal experimental conditions, the linear range was 5—180 ng / mL, the detection limit was 1. 66 ng / mL, with the linear correla-tion coefficient of 0. 992. This immunosensor provides a novel and simple way to detect CRP, and develops the application of the COFs in the electrochemical immunosensor.