CAJ | 학술논문

以镉污染修复植物籽粒苋（Amaranthus hypochondriacus）的根、叶、果为实验材料，利用DDRT-PCR技术分析籽粒苋Cd胁迫下基因表达水平的变化。结果表明：回收差异片段获得镉处理诱导的4个差异表达序列（EST-1、EST-2、EST-3和EST-4），经Blastn比对，EST-1与土人参26S核糖体RNA基因部分序列（GB|HQ843466.1）一致性为99％，EST-2与虎耳草26S核糖体RNA大亚基基因部分序列（GB|AF036498.1）一致性为93％，EST-3与大肠杆菌菌株TW 14359 DNA聚合酶Ⅲα亚基基因（ECs0186）完整CDS（GB|EU906049.1）一致性为97％，EST-4与米曲霉RIB40β-葡萄糖苷酶基因（XM 001816779.2）一致性为99％；经Blastx比对， EST-1与假定蛋白MTR 5g051030[苜蓿]（XP 003614386.1）氨基酸序列相似性为26％，EST-2与假定衰老相关蛋白[柏杉]（GB|ACA30301.1）氨基酸序列一致性为98％，EST-3与DNA聚合酶Ⅲα亚基[大肠杆菌OP50]（ZP 06935700.1）氨基酸序列一致性为100％，EST-4与β-葡萄糖苷酶[黄曲霉NRRL3357]（XP 002383240.1）氨基酸序列一致性为99％；4个差异表达基因通过调控蛋白质代谢、DNA复制和糖代谢等途径来应答镉胁迫。
이력오염수복식물자립현（Amaranthus hypochondriacus）적근、협、과위실험재료，이용DDRT-PCR기술분석자립현Cd협박하기인표체수평적변화。결과표명：회수차이편단획득력처리유도적4개차이표체서렬（EST-1、EST-2、EST-3화EST-4），경Blastn비대，EST-1여토인삼26S핵당체RNA기인부분서렬（GB|HQ843466.1）일치성위99％，EST-2여호이초26S핵당체RNA대아기기인부분서렬（GB|AF036498.1）일치성위93％，EST-3여대장간균균주TW 14359 DNA취합매Ⅲα아기기인（ECs0186）완정CDS（GB|EU906049.1）일치성위97％，EST-4여미곡매RIB40β-포도당감매기인（XM 001816779.2）일치성위99％；경Blastx비대， EST-1여가정단백MTR 5g051030[목숙]（XP 003614386.1）안기산서렬상사성위26％，EST-2여가정쇠로상관단백[백삼]（GB|ACA30301.1）안기산서렬일치성위98％，EST-3여DNA취합매Ⅲα아기[대장간균OP50]（ZP 06935700.1）안기산서렬일치성위100％，EST-4여β-포도당감매[황곡매NRRL3357]（XP 002383240.1）안기산서렬일치성위99％；4개차이표체기인통과조공단백질대사、DNA복제화당대사등도경래응답력협박。
Amaranthus hypochondriacus has great potential for phytoremediation of cadmium(Cd)polluted soils. In this study, differential display reverse transcriptase polymerase chain reaction(DDRT- PCR) was used to screen differentially expressed genes in the roots , leaves and fruits of A . hypochondriacus under Cd stress. The novel bands were recovered and four differentially expressed cDNA fragments [EST (Expressed sequence tags)-1, EST-2, EST-3 and EST-4] were obtained. All ESTs had homology with known cDNA clones in the Genbank databases. The EST-1 was similar to partial sequences of Talinum paniculatum 26S ribosomal RNA gene(GB|HQ843466.1)with an identity of 99%, while the EST-2 showed similarity to partial sequences of Saxifraga mertensiana large subunit 26S ribosomal RNA gene (GB|AF036498.1)with an identity of 93%. The EST-3 was similar to complete cds of Escherichia coli strain TW 14359 DNA poly-merase Ⅲ alpha subunit(ECs0186)gene(GB|EU906049.1)and their identity was 97%. The EST- 4 was similar to Aspergillus oryzae RIB40beta-glucosidaseA(XM 001816779.2)andtheidentitywas99%.AfterBlastXcomparison,EST-1wassimilartohypothetical proteinMTR 5g051030[Medicagotruncatula](XP 003614386.1)withanidentityof26%,EST-2similartoputativesenescence-associat-ed protein[Cupressus sempervirens](GB|ACA30301.1)with an identity of 98%, EST-3 similar to DNA polymerase Ⅲ subunit alpha[Es-cherichiacoliOP50](ZP 06935700.1)withanidentityof100%,andEST-4similartobeta-glucosidase[AspergillusflavusNRRL3357] (XP 002383240.1)withanidentityof99%.Thesefourdifferentiallyexpressedgeneswereresponsivetocadmiumstressthroughregulating protein metabolism, DNA replication and glucose metabolism.