CAJ | 학술논문

目的：观察艾烟与香烟对载脂蛋白E基因敲除(ApoE-/-)小鼠自主行为能力与海马中胶质纤维酸性蛋白(GFAP)表达的影响。方法将13只8周龄C57BL/6小鼠作为空白对照组,27只同龄ApoE-/-小鼠随机分为ApoE-/-模型组、艾烟组、香烟组。香烟组与艾烟组中小鼠暴露于5～15 mg/m3烟气环境。各组小鼠每天干预20 min,每星期6 d,共干预12星期。于第13星期进行行为学测试,之后处死动物、取材,对脑组织海马中GFAP进行免疫组化法检测。结果空白组小鼠自主活动明显高于模型组小鼠自主活动(P＜0.05)；香烟组没有表现出与模型组、艾烟组的明显差异(P＞0.05),但低于空白组(P＜0.05)。艾烟组移动距离高于模型组(P＜0.05),站立次数低于空白组。模型组小鼠海马中GFAP免疫反应产物积分光密度明显高于空白组与艾烟组(P＜0.05)。香烟组小鼠海马GFAP表达高于艾烟组与空白组(P＜0.05)。结论艾烟可能增强小鼠中枢神经系统的兴奋性,减低阿茨海默病模型小鼠海马中GFAP的表达。
목적：관찰애연여향연대재지단백E기인고제(ApoE-/-)소서자주행위능력여해마중효질섬유산성단백(GFAP)표체적영향。방법장13지8주령C57BL/6소서작위공백대조조,27지동령ApoE-/-소서수궤분위ApoE-/-모형조、애연조、향연조。향연조여애연조중소서폭로우5～15 mg/m3연기배경。각조소서매천간예20 min,매성기6 d,공간예12성기。우제13성기진행행위학측시,지후처사동물、취재,대뇌조직해마중GFAP진행면역조화법검측。결과공백조소서자주활동명현고우모형조소서자주활동(P＜0.05)；향연조몰유표현출여모형조、애연조적명현차이(P＞0.05),단저우공백조(P＜0.05)。애연조이동거리고우모형조(P＜0.05),참립차수저우공백조。모형조소서해마중GFAP면역반응산물적분광밀도명현고우공백조여애연조(P＜0.05)。향연조소서해마GFAP표체고우애연조여공백조(P＜0.05)。결론애연가능증강소서중추신경계통적흥강성,감저아자해묵병모형소서해마중GFAP적표체。
ObjectiveTo investigate the effects of moxa smoke versus tobaccosmoke on autonomous behaviors and hippocampal expression of glial fibrillary acidic protein (GFAP) in apolipoprotein E gene knockout (ApoE-/-) mice.Method Thirteen 8-week-old C57BL/6 mice constituted a blank control group. Twenty-seven ApoE-/-mice of the same age were randomized into ApoE-/-model, moxa smoke and tobacco smoke groups. The tobacco smoke and moxa smoke groups of mice were exposed to smoke 5-15 mg/m3circumstances. Every group of mice was intervened in 20 min. daily, six days a week, for atotal of 12 weeks. A behavioral test was conducted in week 13. The animals were then sacrificed to take the materials. Hippocampal GFAP in the brain was measured by an immunohistochemical method.ResultAutonomous activities were significantly more in theblank group than in the model group (P<0.05) and significantly fewer in the tobacco smoke group than in the blank group (P<0.05). There was no statistically significant difference in autonomous activities between the tobacco smoke group and the model or moxa smoke group (P>0.05). Moving distance was longer in the moxa smoke group than in the model group (P<0.05). Standing-up number was smaller in the moxa smoke group than in the blank group (P<0.05). Integral optical density of GFAP immune reaction products in the hippocampus was significantlyhigher in the model group of mice than in the blank and moxa smoke groups (P<0.05). Hippocampal GFAP expression was significantly higher in the group of mice than in the moxa smoke and blank groups (P<0.05).Conclusion Moxa smoke can increase the excitability of central nervous system in mice and reduce hippocampal GFAP expression in a mice model of Alzheimer disease.