CAJ | 학술논문

为探讨Sr2+污染对蚕豆根尖细胞的生态毒性效应和对植物生长影响的毒理机制，以蚕豆为试材，通过急性毒性实验、微核实验和彗星实验，以ρ（Sr2+）0.01 mmol·L-1处理为CK，分析了Sr2+处理浓度[ρ（Sr2+）]为0.05~10 mmol·L-1对蚕豆根（芽）生长、干重、根尖细胞染色体结构及根尖细胞DNA断裂损伤的影响。结果显示，Sr2+对蚕豆的根尖细胞的生态毒性效应具有双重性。当ρ（Sr2+）<0.05 mmol·L-1时可促进蚕豆根、芽生长；ρ（Sr2+）>0.25 mmol·L-1时，蚕豆发芽率下降了17.93％~36.32％，根茎、生物量及总生物量分别降低了42.15％、48.69％和46.75％。同时，Sr2+抑制了蚕豆根尖细胞有丝分裂，促进DNA链发生断裂，导致有丝分裂指数（MI）降低，出现染色体断片和微核，其微核率（MCN）是CK的200％~400％；彗星尾长（TL）、尾部DNA含量（TD）和彗星尾矩（TM）也显著高于CK（P<0.05），表明DNA出现了明显的断裂损伤。
위탐토Sr2+오염대잠두근첨세포적생태독성효응화대식물생장영향적독리궤제，이잠두위시재，통과급성독성실험、미핵실험화혜성실험，이ρ（Sr2+）0.01 mmol·L-1처리위CK，분석료Sr2+처리농도[ρ（Sr2+）]위0.05~10 mmol·L-1대잠두근（아）생장、간중、근첨세포염색체결구급근첨세포DNA단렬손상적영향。결과현시，Sr2+대잠두적근첨세포적생태독성효응구유쌍중성。당ρ（Sr2+）<0.05 mmol·L-1시가촉진잠두근、아생장；ρ（Sr2+）>0.25 mmol·L-1시，잠두발아솔하강료17.93％~36.32％，근경、생물량급총생물량분별강저료42.15％、48.69％화46.75％。동시，Sr2+억제료잠두근첨세포유사분렬，촉진DNA련발생단렬，도치유사분렬지수（MI）강저，출현염색체단편화미핵，기미핵솔（MCN）시CK적200％~400％；혜성미장（TL）、미부DNA함량（TD）화혜성미구（TM）야현저고우CK（P<0.05），표명DNA출현료명현적단렬손상。
Strontium(Sr2+)in the environment easily enters plants and poses health risks to human via food pathway. This study was per-formed to investigate the genotoxic effects of Sr2+on the root tip cells of broad bean(Vicia faba L.), a Sr2+high-accumulating and sensitive plant, through acute toxicity test, micronucleus assay, and comet assay. The germination rate, seedling dry weight, chromosome breakage, and DNA damage of broad bean were examined at different Sr2+concentrations [ρ(Sr2+)0.05~10 mmol·L-1] for 24 h, 48 h, and 72 h, withρ(Sr2+)0.01 mmol·L-1 as control(CK). Low concentrations of Sr2+[ρ(Sr2+)<0.05 mmol·L-1] promoted elongation of the root and shoot of broad bean, but the germination rates were reduced by up to 17.93%~36.32% by ρ(Sr2+)> 0.25 mmol·L-1. As Sr2+concentrations wereρ(Sr2+)0.25~10 mmol·L-1, the average biomass and the mitotic index(MI)of the plant decreased, while the chromosomal aberration fre-quency(CAF), the micronucleus frequency(MCN)and the three parameter values of the comet assay(tail length, tail DNA%, and tail mo-ment)all increased. In conclusion, Sr2+has a dual effect on root tip cells, depending upon its concentration levels. The toxicity mechanism of Sr2+on broad bean is structural damages of DNA in root tip cells.