农业环境科学学报
農業環境科學學報
농업배경과학학보
Journal of Agro-Environment Science
2015年
9期
1640-1645
,共6页
邓鑫%周祥%刘志红%李晓军%台培东
鄧鑫%週祥%劉誌紅%李曉軍%檯培東
산흠%주상%류지홍%리효군%태배동
伸展摇蚊%黄色羽摇蚊%Cd2+%毒性试验%LC50
伸展搖蚊%黃色羽搖蚊%Cd2+%毒性試驗%LC50
신전요문%황색우요문%Cd2+%독성시험%LC50
Chironomus riparius%Chironomus flaviplumus%Cd2+%toxicity test%LC50
依据《化学品沉积物-水系统中摇蚊毒性试验加标于沉积物法》(GB/T 27859—2011),以死亡率、羽化率、羽化时间和口器畸形率为观测指标,分别研究了加标水体和沉积物中 Cd2+(CdCl2·2.5H2O)对伸展摇蚊(Chironomus riparius)和黄色羽摇蚊(Chironomus flaviplumus)的急性(96 h)和慢性(20 d)毒性。结果表明,摇蚊幼虫存活率与水和沉积物中Cd2+浓度呈显著负相关(P<0.01),水中Cd2+对三龄伸展摇蚊幼虫和黄色羽摇蚊幼虫的96 h半致死浓度(LC50)分别为22.39 mg·L-1和33.41 mg·L-1,沉积物中Cd2+对伸展摇蚊幼虫及黄色羽摇蚊幼虫的20 d LC50分别为226.26 mg·kg-1和351.84 mg·kg-1。摇蚊幼虫口器畸形率与Cd2+浓度显著正相关(P<0.01);伸展摇蚊羽化时间与Cd2+浓度无显著相关关系,黄色羽摇蚊羽化时间与Cd2+浓度显著正相关(P<0.05)。死亡率和口器畸形率表明,伸展摇蚊对镉污染的敏感性要高于黄色羽摇蚊。
依據《化學品沉積物-水繫統中搖蚊毒性試驗加標于沉積物法》(GB/T 27859—2011),以死亡率、羽化率、羽化時間和口器畸形率為觀測指標,分彆研究瞭加標水體和沉積物中 Cd2+(CdCl2·2.5H2O)對伸展搖蚊(Chironomus riparius)和黃色羽搖蚊(Chironomus flaviplumus)的急性(96 h)和慢性(20 d)毒性。結果錶明,搖蚊幼蟲存活率與水和沉積物中Cd2+濃度呈顯著負相關(P<0.01),水中Cd2+對三齡伸展搖蚊幼蟲和黃色羽搖蚊幼蟲的96 h半緻死濃度(LC50)分彆為22.39 mg·L-1和33.41 mg·L-1,沉積物中Cd2+對伸展搖蚊幼蟲及黃色羽搖蚊幼蟲的20 d LC50分彆為226.26 mg·kg-1和351.84 mg·kg-1。搖蚊幼蟲口器畸形率與Cd2+濃度顯著正相關(P<0.01);伸展搖蚊羽化時間與Cd2+濃度無顯著相關關繫,黃色羽搖蚊羽化時間與Cd2+濃度顯著正相關(P<0.05)。死亡率和口器畸形率錶明,伸展搖蚊對鎘汙染的敏感性要高于黃色羽搖蚊。
의거《화학품침적물-수계통중요문독성시험가표우침적물법》(GB/T 27859—2011),이사망솔、우화솔、우화시간화구기기형솔위관측지표,분별연구료가표수체화침적물중 Cd2+(CdCl2·2.5H2O)대신전요문(Chironomus riparius)화황색우요문(Chironomus flaviplumus)적급성(96 h)화만성(20 d)독성。결과표명,요문유충존활솔여수화침적물중Cd2+농도정현저부상관(P<0.01),수중Cd2+대삼령신전요문유충화황색우요문유충적96 h반치사농도(LC50)분별위22.39 mg·L-1화33.41 mg·L-1,침적물중Cd2+대신전요문유충급황색우요문유충적20 d LC50분별위226.26 mg·kg-1화351.84 mg·kg-1。요문유충구기기형솔여Cd2+농도현저정상관(P<0.01);신전요문우화시간여Cd2+농도무현저상관관계,황색우요문우화시간여Cd2+농도현저정상관(P<0.05)。사망솔화구기기형솔표명,신전요문대력오염적민감성요고우황색우요문。
Chironomidae are important organisms indicating the quality of water environment. Here acute(96 h)and chronic(20 d)toxicity experiments were carried out to determine the toxicity of Cd2+on the larvae of Chironomus riparius and Chironomus flaviplumus, following the standard methods in the guideline GB/T 27859—2011(Chemicals-Sediment-water chironomid toxicity test:Spiked sediment method). The mortality, emergence rate, emergence time, and mentum deformity rate were observed. The 96 h LC50 values of cadmium to three instar C. riparius and C. flaviplumus larvae were 22.39 mg·L-1 and 33.41 mg·L-1, respectively. The 20 d LC50 values of Cd to C. riparius and C. flaviplumus were correspondingly 226.26 mg·kg-1 and 351.84 mg·kg-1. Their survival rate and mentum deformity rate were significantly positive correlated to Cd2+concentration(P<0.01). Emergence time of C. riparius was not significantly affected by Cd2+in the sediment, whereas that of C. flaviplumus was significantly positively correlated to Cd2+concentration(P<0.05). However, the mortality and mentum de-formity rates indicated that C. riparius was more sensitive to cadmium than C. flaviplumus.