亚太热带生物医学杂志（英文版）
亞太熱帶生物醫學雜誌（英文版）
아태열대생물의학잡지（영문판）
Asian pacific Journal of Tropical Biomedicine
2012年
6期
469-473
,共5页
Marine Actinomycetes%Antimicrobial activity%Streptomyces%16S rRNA%Cross-streak method%Antibacterial substance%Kirby-Bauer disc diffusion method%Molecular identification%Morphological character
Objective:To evaluate the antimicrobial activity of Actinomycetes species isolated from marine environment. Methods: Twenty one strains of Actinomycetes were isolated from samples of Royapuram, Muttukadu, Mahabalipuram sea shores and Adyar estuary. Preliminary screening was done using cross-streak method against two gram-positive and eight gram-negative bacteria. The most potent strains C11 and C12 were selected from which antibacterial substances were extracted. The antibacterial activities of the extracts were performed using Kirby-Bauer disc diffusion method. Molecular identification of those isolates was done. Results:All those twenty one isolates were active against at least one of the test organisms. Morphological characters were recorded. C11 showed activity against Staphylococcus species (13.0±0.5 mm), Vibrio harveyi (11.0±0.2 mm), Pseudomonas species (12.0±0.3 mm). C12 showed activity against Staphylococcus species (16.0±0.4 mm), Bacillus subtilis (11.0±0.2 mm), Vibrio harveyi (9.0±0.1 mm), Pseudomonas species (10.0±0.2 mm). 16S rRNA pattern strongly suggested that C11 and C12 strains were Streptomyces species. Conclusions: The results of the present investigation reveal that the marine Actinomycetes from coastal environment are the potent source of novel antibiotics. Isolation, characterization and study of Actinomycetes can be useful in discovery of novel species of Actinomycetes.
