东北林业大学学报
東北林業大學學報
동북임업대학학보
Journal of Northeast Forestry University
2015年
9期
34-40
,共7页
大岩桐%花芽分化%试管开花
大巖桐%花芽分化%試管開花
대암동%화아분화%시관개화
Sinningia speciosa%Floral bud differentiation%in vitro flowering
以大岩桐（ Sinningias peciosa）试管苗为试验材料，研究了基本培养基种类、植物生长调节剂种类、培养基不同成分、赤霉素质量浓度及植株营养生长状态对诱导试管内花芽分化的影响。结果表明，WPM培养基是适宜大岩桐诱导花芽分化的基本培养基种类；适宜的细胞分裂素6－BA与生长素 NAA质量浓度配比，对提高大岩桐花芽分化率较为有利；诱导大岩桐试管内花芽分化的适宜培养基为： WPM＋6－BA 0．3mg · L－1＋NAA0．2 mg· L－1＋蔗糖40 g· L－1＋琼脂9 g· L－1；赤霉素不利于健壮生长。3 cm的苗径大小是大岩桐营养生长向生殖生长转化的临界条件，达到临界条件后，花芽分化率随苗径增加而逐渐增多。
以大巖桐（ Sinningias peciosa）試管苗為試驗材料，研究瞭基本培養基種類、植物生長調節劑種類、培養基不同成分、赤黴素質量濃度及植株營養生長狀態對誘導試管內花芽分化的影響。結果錶明，WPM培養基是適宜大巖桐誘導花芽分化的基本培養基種類；適宜的細胞分裂素6－BA與生長素 NAA質量濃度配比，對提高大巖桐花芽分化率較為有利；誘導大巖桐試管內花芽分化的適宜培養基為： WPM＋6－BA 0．3mg · L－1＋NAA0．2 mg· L－1＋蔗糖40 g· L－1＋瓊脂9 g· L－1；赤黴素不利于健壯生長。3 cm的苗徑大小是大巖桐營養生長嚮生殖生長轉化的臨界條件，達到臨界條件後，花芽分化率隨苗徑增加而逐漸增多。
이대암동（ Sinningias peciosa）시관묘위시험재료，연구료기본배양기충류、식물생장조절제충류、배양기불동성분、적매소질량농도급식주영양생장상태대유도시관내화아분화적영향。결과표명，WPM배양기시괄의대암동유도화아분화적기본배양기충류；괄의적세포분렬소6－BA여생장소 NAA질량농도배비，대제고대암동화아분화솔교위유리；유도대암동시관내화아분화적괄의배양기위： WPM＋6－BA 0．3mg · L－1＋NAA0．2 mg· L－1＋자당40 g· L－1＋경지9 g· L－1；적매소불리우건장생장。3 cm적묘경대소시대암동영양생장향생식생장전화적림계조건，체도림계조건후，화아분화솔수묘경증가이축점증다。
With aseptic plantlets of Sinningia speciosa, we studied the effects of basic mediums , plant growth regulators , differ-ent concentrations of mineral , 6-BA, NAA, sugar and agar , concentrations of gibberellin , and plantlet physiological sta-tus on in vitro flowering.The most suitable basic mediums was WPM , while the combination of cytokinin 6-BA and auxin NAA was suitable for flower bud differentiation of S.speciose.WPM medium with 6-BA 0.3 mg· L-1 , NAA 0.2 mg· L-1 , sugar 40 g· L-1 and agar 9 g· L-1 was suitable for inducing in vitro flower buds differentiation .Gibberellin made against with vigorous growth .The 3-cm seedling diameter of the plantlets was the critical condition of the transition from vegetative to reproductive growth for S.speciosa, and with the increase of the plantlet seedling diameter , the percentage of floral bud differentiation was increased .
