CAJ | 학술논문

利用RT－PCR结合RACE的方法从东方百合‘索邦’花被片中克隆了查尔酮异构酶（CHI）基因，命名为LhCHI（GenBank登录号为KJ784468）。该基因开放阅读框702 bp，编码233个氨基酸，预测该蛋白相对分子质量25 KD，等电点（ pI）为4．7。同源比对和系统进化分析表明，LhCHI基因编码的氨基酸序列具有查尔酮异构酶典型的催化活性保守位点，与百合科郁金香（ Tulipa fosteriana）查尔酮异构酶序列一致性为83．3％。半定量PCR和荧光实时定量PCR分析结果表明，LhCHI基因在百合的根、茎、叶片、鳞茎、开放花被片、花药以及柱头中均有表达，花器官中相对表达量较高，花发育后期的柱头、花柱、花被片等组织中LhCHI基因表达水平普遍高于花发育早期。
이용RT－PCR결합RACE적방법종동방백합‘색방’화피편중극륭료사이동이구매（CHI）기인，명명위LhCHI（GenBank등록호위KJ784468）。해기인개방열독광702 bp，편마233개안기산，예측해단백상대분자질량25 KD，등전점（ pI）위4．7。동원비대화계통진화분석표명，LhCHI기인편마적안기산서렬구유사이동이구매전형적최화활성보수위점，여백합과욱금향（ Tulipa fosteriana）사이동이구매서렬일치성위83．3％。반정량PCR화형광실시정량PCR분석결과표명，LhCHI기인재백합적근、경、협편、린경、개방화피편、화약이급주두중균유표체，화기관중상대표체량교고，화발육후기적주두、화주、화피편등조직중LhCHI기인표체수평보편고우화발육조기。
The gene LhCHI encoding chalcone isomerase (CHI) involved into flavonoids synthesis was cloned from inner tepals of Oriental hybrid lily ‘Sorbonne ’ ( LhCHI, GenBank accession No .KJ784468 ) .The cDNA of LhCHI was obtained by RT-PCR and Rapid Amplification of cDNA Ends techniques .The open reading frame of LhCHI was 702 bp in length, enco-ding a protein polypeptide of 233 amino acids with a predicted molecular weight of 25 kD and a theoretical pI of 4.7.The deduced amino acid sequence of LhCHI shared 83.3%identity with CHI from Tulipa fosteriana, and contained typical con-served catalytic chalcone elements .Quantitative real-time PCR analyzed the expression profiles of LhCHI in different tis-sues.LhCHI was constitutively expressed in root , stem, leaf, bulb, tepal, anther, style and stigma with particularly high expression in flowers .The expression level of LhCHI in later stage flowers is generally higher than that in the early stage flowers.