CAJ | 학술논문

为探究欧美杂交杨转录调控因子 Pnd－WRKY3在落叶松－杨栅锈菌（ Melam pos ra larici-populina） E4生理小种侵染欧美杨（Populsu nigra×P．deltoides）后不同时间点的具体功能，根据转录组测序结果设计特异性引物进行Pnd－WRKY3基因CDS区克隆，应用生物信息学预测Pnd－WRKY3调控的靶基因，采用荧光定量Q－PCR研究Pnd－WRKY3基因抗锈菌表达特性。结果表明：Pnd－WRKY3基因蛋白编码区全长1779 bp，预测具有W盒的靶基因49条，其中杨树动力蛋白表达量与Pnd－WRKY3基因表达量正相关。 Q－PCR 结果表明，锈菌菌丝生长期及生物量大量增长时期，Pnd－WRKY3基因表达量上调。在锈菌完成一个生长周期形成夏孢子时，Pnd－WRKY3基因表达量开始下降。
위탐구구미잡교양전록조공인자 Pnd－WRKY3재락협송－양책수균（ Melam pos ra larici-populina） E4생리소충침염구미양（Populsu nigra×P．deltoides）후불동시간점적구체공능，근거전록조측서결과설계특이성인물진행Pnd－WRKY3기인CDS구극륭，응용생물신식학예측Pnd－WRKY3조공적파기인，채용형광정량Q－PCR연구Pnd－WRKY3기인항수균표체특성。결과표명：Pnd－WRKY3기인단백편마구전장1779 bp，예측구유W합적파기인49조，기중양수동력단백표체량여Pnd－WRKY3기인표체량정상관。 Q－PCR 결과표명，수균균사생장기급생물량대량증장시기，Pnd－WRKY3기인표체량상조。재수균완성일개생장주기형성하포자시，Pnd－WRKY3기인표체량개시하강。
In order to reveal the resistance molecular mechanism of the hybrid poplar Robusta ( Popul us nigra×P.deltoides) to the E4 virulent filial generation of Melampsora laric-populina, we cloned the Pnd-WRKY3 transcription factor gene , and studied the gene function of Pnd-WRKY3 during the rust infection .The complete CDS of Pnd-WRKY3 was 1 779 bp.Then, we predicted 49 target genes of Pnd-WRKY3 with the W box, including one dynamin protein gene , in which expression quantity had positive correlation with that of Pnd-WRKY3.By Q-PCR, the expression of Pnd-WRKY3 was promoted during rust mycelial growth period and the reproductive period .The expression was suppressed when spores were formed , then the life cycle of rust was finished at the same stage .