CAJ | 학술논문

目的探讨通过股动脉注射神经肽Y建立冠状动脉微血管痉挛小型猪模型的可行性,并分析此模型的特征.方法将实验猪分为4组:生理盐水组、1 nmol神经肽Y组、3 nmol神经肽Y组和6 nmol神经肽Y组(均为4例).经双侧股动脉送入动脉鞘管,左侧动脉鞘管送入猪尾导管,测定血液动力学指标.通过右侧动脉鞘管送入导管,根据分组情况在左前降支中段注射不同剂量的神经肽Y和生理盐水,分别在注射前、注射后10和30 min采用心肌声学造影测定微血管容积(α)、充填速率(β)和微循环血流量(MBF).结果生理盐水组和1 nmol神经肽Y组注射前后的仅、β和MBF差异均无统计学意义(P均＞0.05).3 nmol神经肽Y组在注射后10 min,α和MBF均低于注射前(P分别＜0.01和0.05);注射30 min后,α、β和MBF均有不同程度升高,其中α与注射前比较差异仍有统计学意义(P＜0.05).6 nmol神经肽Y组在注射后10 min,β和MBF均低于注射前(P分别＜0.05和0.01);注射30 min后,α、β和MBF均有不同程度升高,其中MBF与注射前比较差异仍有统计学意义(P＜0.01),且小于3 nmol神经肽Y组(P＜0.05).结论小型猪左前降支中段注射神经肽Y可建立冠状动脉微血管痉挛动物模型,该模型符合临床发病特点,有一定的实用价值.
목적 탐토통과고동맥주사신경태Y건립관상동맥미혈관경련소형저모형적가행성,병분석차모형적특정.방법 장실험저분위4조:생리염수조、1 nmol신경태Y조、3 nmol신경태Y조화6 nmol신경태Y조(균위4례).경쌍측고동맥송입동맥초관,좌측동맥초관송입저미도관,측정혈액동역학지표.통과우측동맥초관송입도관,근거분조정황재좌전강지중단주사불동제량적신경태Y화생리염수,분별재주사전、주사후10화30 min채용심기성학조영측정미혈관용적(α)、충전속솔(β)화미순배혈류량(MBF).결과 생리염수조화1 nmol신경태Y조주사전후적부、β화MBF차이균무통계학의의(P균＞0.05).3 nmol신경태Y조재주사후10 min,α화MBF균저우주사전(P분별＜0.01화0.05);주사30 min후,α、β화MBF균유불동정도승고,기중α여주사전비교차이잉유통계학의의(P＜0.05).6 nmol신경태Y조재주사후10 min,β화MBF균저우주사전(P분별＜0.05화0.01);주사30 min후,α、β화MBF균유불동정도승고,기중MBF여주사전비교차이잉유통계학의의(P＜0.01),차소우3 nmol신경태Y조(P＜0.05).결론 소형저좌전강지중단주사신경태Y가건립관상동맥미혈관경련동물모형,해모형부합림상발병특점,유일정적실용개치.
Objective To observe the feasibility of establishing a porcine model of microvascular coronary artery spasm by intracoronary neuropeptide Y(NPY) infusion,and to analyze the characterization of this model.Methods Minipigs were divided into four groups (n =4 each):normal saline (NS) group,1 nmol NPY group,3 nmol NPY group,and 6 nmol NPY group.Arterial sheaths were planted into bilateral femoral arteries of minipigs.A pigtail catheter was placed at the left sheath to determine the hemodynamic parameters.NS and different doses of NPY were injected into the left anterior descending branch through the right sheath.Intravenous myocardial contrast echocardiography (MCE) was applied to measure the microvessel volume(α),filling velocity(β),and microcirculation blood flow (MBF) before and at 10 and 30 minutes after NS and NPY injection.Results Before and after injection,there were no difference in α,β andMBF between NS and 1 nmol NPY group(all P ＞0.05).In 3 nmol NPY group,α and MBF decreased at 10 min (P ＜ 0.01 and 0.05,respectively),which were recovered at 30 min except α.Reductions of β(P ＜0.05)and MBF (P ＜0.01)were observed at 10 min in 6 nmol NPY group,which were recovered at 30 min,but MBF still remained lower than at baseline(P ＜0.01)and compared to 3 nmol NPY group (P ＜ 0.05).Conclusion Intracoronary injection of NPY into the anterior descending coronary artery can establish the porcine model of microvascular coronary artery spasm,which might serve as a useful animal model for coronary microvascular studies.