CAJ | 학술논문

目的：观察龙牡二妙汤联合复方氟米松软膏治疗湿疹的临床疗效及可能机制。方法选取2012年3月-2014年5月的327例湿疹患者进行回顾性分析，随机分为3组，即龙牡二妙汤组（A 组）113例，复方氟米松软膏组（B 组）109例以及龙牡二妙汤联合复方氟米松软膏治疗组（ C 组）105例。对比3组患者临床疗效及治疗前后的皮损与瘙痒评分。另外，取患者皮损组织，ELISA 法检测 Th1和 Th2各细胞因子的含量，流式细胞术检测 INF -γ、IL -4表达情况。结果治疗后 C 组临床疗效明显优于 A、B 组，差异有统计学意义（P ﹤0.05，P ﹤0.01）；治疗后 C 组皮损与瘙痒评分均显著低于 A、B 组，差异有统计学意义（P ﹤0.05，P ﹤0.01）；治疗后 C 组皮损组织的 IL -2、INF -γ水平显著高于 A、B 组，IL -4、IL -10、IgE 水平显著低于 A、B 组，差异有统计学意义（P ﹤0.05，P ﹤0.01）。流式细胞术检测结果发现 C 组 INF -γ呈显著升高，而 IL -4显著降低，差异有统计学意义（P ﹤0.05，P
목적：관찰룡모이묘탕연합복방불미송연고치료습진적림상료효급가능궤제。방법선취2012년3월-2014년5월적327례습진환자진행회고성분석，수궤분위3조，즉룡모이묘탕조（A 조）113례，복방불미송연고조（B 조）109례이급룡모이묘탕연합복방불미송연고치료조（ C 조）105례。대비3조환자림상료효급치료전후적피손여소양평분。령외，취환자피손조직，ELISA 법검측 Th1화 Th2각세포인자적함량，류식세포술검측 INF -γ、IL -4표체정황。결과치료후 C 조림상료효명현우우 A、B 조，차이유통계학의의（P ﹤0.05，P ﹤0.01）；치료후 C 조피손여소양평분균현저저우 A、B 조，차이유통계학의의（P ﹤0.05，P ﹤0.01）；치료후 C 조피손조직적 IL -2、INF -γ수평현저고우 A、B 조，IL -4、IL -10、IgE 수평현저저우 A、B 조，차이유통계학의의（P ﹤0.05，P ﹤0.01）。류식세포술검측결과발현 C 조 INF -γ정현저승고，이 IL -4현저강저，차이유통계학의의（P ﹤0.05，P
Objective To observe the clinical efficacy and potential mechanism of the combined therapy of longmu ermiao decoction and compound flumetasone ointment in treatment of eczema. Methods Three hundred and twenty - seven eczema patients were collected for retrospective analysis from March 2012 to May 2014 and were randomized into 3 groups,named A group(longmu ermiao decoction)(113 cases),B group(compound flumetasone ointment)(109 cases)and C group(the combined therapy of longmu ermiao decoction and compound flumetasone ointment)(105 cases). The clinical efficacy and the scores of skin le-sion and pruritus were compared before and after treatment. Additionally,taking skin lesion tissue,ELISA was used to determine the contents of Th1 and Th2 cytokines;the flow cytometry was applied to the expression of INF - γ and IL - 4. Results After treatment,the clinical efficacy in the C group was apparently better than that in the A and B groups,indicating the significant difference(P ﹤ 0. 05,P ﹤ 0. 01). After treatment,the scores of skin lesion and pruritus in the C group were lower significantly than those in the A and B group,in-dicating the significant difference(P ﹤ 0. 05,P ﹤ 0. 01). After treatment,the levels of INF - γ and IL - 4 in the skin lesion tissue were higher significantly than those in the A and B group and the levels of IL - 4,IL -10 and IgE were lower significantly than those in the A and B group,indicating the significant difference(P ﹤0. 05,P ﹤ 0. 01). The flow cytometry results showed that INF - γ was increased significantly and IL - 4 was reduced significantly,indicating the significant difference(P ﹤ 0. 05,P ﹤ 0. 01). Conclusion The combined therapy of longmu ermiao decoction and compound flumetasone ointment achieves the better efficacy on eczema compared with simple application of longmu ermiao decoction or compound flumetasone ointment. The potential mechanism is that longmu ermiao decoction rectifies the dysfunction of Th1 / Th2 cells in the patients.