世界中西医结合杂志
世界中西醫結閤雜誌
세계중서의결합잡지
World Journal of Integrated Traditional and Western Medicine
2015年
9期
1303-1305
,共3页
刘芳芳%王平%陶功定%李俊莲%邱继云
劉芳芳%王平%陶功定%李俊蓮%邱繼雲
류방방%왕평%도공정%리준련%구계운
脾阳虚%内外合邪%潮湿环境%脾细胞增殖
脾暘虛%內外閤邪%潮濕環境%脾細胞增殖
비양허%내외합사%조습배경%비세포증식
Spleen yang deficiency%Internal and external pathogenic%Humid environment%Spleen cell proliferation
目的：探讨外湿环境因素对正常及脾阳虚大鼠脾细胞增殖能力的影响，研究复合病因内外合邪发病机制。方法选取 SD 大鼠40只随机分为对照组、外湿组、脾阳虚组、脾阳虚加湿组，每组10只。造模成功后无菌摘除脾脏，采用 ConA 诱导脾淋巴细胞增殖，以此表示淋巴细胞的功能水平。结果脾阳虚组、外湿组、脾阳虚加湿组脾淋巴细胞增殖能力均显著低于对照组，差异有统计学意义（P ﹤0.01）。脾阳虚加湿组脾淋巴细胞增殖能力较脾阳虚组显著下降，差异有统计学意义（ P ﹤0.01）。结论外湿环境因素相对于脾阳虚因素对机体免疫抑制作用更强。说明外湿环境因素对正常大鼠、脾阳虚模型大鼠免疫学的影响较单纯脾阳虚模型大鼠明显，并且外湿环境因素与脾阳虚模型具有协同致免疫机能下降的作用。
目的：探討外濕環境因素對正常及脾暘虛大鼠脾細胞增殖能力的影響，研究複閤病因內外閤邪髮病機製。方法選取 SD 大鼠40隻隨機分為對照組、外濕組、脾暘虛組、脾暘虛加濕組，每組10隻。造模成功後無菌摘除脾髒，採用 ConA 誘導脾淋巴細胞增殖，以此錶示淋巴細胞的功能水平。結果脾暘虛組、外濕組、脾暘虛加濕組脾淋巴細胞增殖能力均顯著低于對照組，差異有統計學意義（P ﹤0.01）。脾暘虛加濕組脾淋巴細胞增殖能力較脾暘虛組顯著下降，差異有統計學意義（ P ﹤0.01）。結論外濕環境因素相對于脾暘虛因素對機體免疫抑製作用更彊。說明外濕環境因素對正常大鼠、脾暘虛模型大鼠免疫學的影響較單純脾暘虛模型大鼠明顯，併且外濕環境因素與脾暘虛模型具有協同緻免疫機能下降的作用。
목적：탐토외습배경인소대정상급비양허대서비세포증식능력적영향，연구복합병인내외합사발병궤제。방법선취 SD 대서40지수궤분위대조조、외습조、비양허조、비양허가습조，매조10지。조모성공후무균적제비장，채용 ConA 유도비림파세포증식，이차표시림파세포적공능수평。결과비양허조、외습조、비양허가습조비림파세포증식능력균현저저우대조조，차이유통계학의의（P ﹤0.01）。비양허가습조비림파세포증식능력교비양허조현저하강，차이유통계학의의（ P ﹤0.01）。결론외습배경인소상대우비양허인소대궤체면역억제작용경강。설명외습배경인소대정상대서、비양허모형대서면역학적영향교단순비양허모형대서명현，병차외습배경인소여비양허모형구유협동치면역궤능하강적작용。
Objective To investigate the effect of external humidity environment factors on the pro-liferation of spleen cells in normal and spleen deficiency rats,and to study the pathogenesis of internal and external pathogenic mechanisms of the compound. Methods Forty SD rats were randomly divided into the control group,the wet group,the spleen yang deficiency group,and the Spleen Yang humidification group. There were 10 rats in each group. The spleen was successfully removed after the model was successfully made,and the spleen lymphocyte proliferation was induced by ConA,so as to express the function of the lym-phocyte. Results The spleen lymphocyte proliferation ability of spleen deficiency group,external dampness group and spleen yang humidification group were significantly lower than that of the control group( P ﹤0. 01). The spleen lymphocyte proliferation ability of spleen deficiency and spleen deficiency humidification group were significantly decreased( P ﹤ 0. 01). Conclusion The external humidity environment factor is stronger than the spleen yang deficiency factor to the immune suppression effect of organism. Wet environment factors shows more obvious immunological effect in normal rats and spleen yang deficiency model rats than simple spleen yang deficiency model rats. And wet environment factors and spleen yang deficiency model have collaborative induced decline effect in immune function.
