中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
Chinese Journal of Industrial Hygiene and Occupational Diseases
2015年
9期
682-684
,共3页
郝莉%贺金奖%张磊%郭建峰%齐娜娜%杨章萍%张旭慧%杨洛贤%张增利
郝莉%賀金獎%張磊%郭建峰%齊娜娜%楊章萍%張旭慧%楊洛賢%張增利
학리%하금장%장뢰%곽건봉%제나나%양장평%장욱혜%양락현%장증리
挥发性有机物%睾酮%雌二醇%乳酸脱氢酶
揮髮性有機物%睪酮%雌二醇%乳痠脫氫酶
휘발성유궤물%고동%자이순%유산탈경매
Volatile Organic Compounds%Testosterone%Estradiol%Lactate dehydrogenase
目的 研究多种挥发性有机物(VOCs)对小鼠睾丸组织相关酶及激素的影响.方法 急性毒性试验确定VOCs的LD50后,选择清洁级昆明种纯系雄性小鼠40只,分为1/8 LD50 VOCs染毒组、1/4 LD50VOCs染毒组、1/2 LD50 VOCs染毒组、环磷酰胺(60 mg/kg)阳性对照组和茶油阴性对照组,每组8只,连续腹腔注射5d,应用ELISA法测定小鼠睾丸组织中激素睾酮(T)、雌二醇(E2)、卵泡刺激素(FSH)、促黄体生成素(LH)水平,同步测定乳酸脱氢酶(LDH)、γ-谷氨酸转肽酶(γ-GT)、酸性磷酸酶(ACP)、葡萄糖-6-磷酸脱氢酶(G-6-PD)的活力.结果 与阴性对照组比较,1/8LD50 VOCs染毒组小鼠睾丸系数明显增高,差异有统计学意义(P＜0.05).各染毒组的睾丸标志酶活力随着染毒剂量的增加呈现下降趋势,与阴性对照组比较,差异有统计学意义(P＜0.05).各染毒组的睾丸性激素水平随着染毒剂量的增加呈现下降趋势,与阴性对照组比较,差异有统计学意义(P＜0.05).结论 VOCs对雄性小鼠睾丸具有明显的毒性,可影响其睾丸性激素水平和酶活力.
目的 研究多種揮髮性有機物(VOCs)對小鼠睪汍組織相關酶及激素的影響.方法 急性毒性試驗確定VOCs的LD50後,選擇清潔級昆明種純繫雄性小鼠40隻,分為1/8 LD50 VOCs染毒組、1/4 LD50VOCs染毒組、1/2 LD50 VOCs染毒組、環燐酰胺(60 mg/kg)暘性對照組和茶油陰性對照組,每組8隻,連續腹腔註射5d,應用ELISA法測定小鼠睪汍組織中激素睪酮(T)、雌二醇(E2)、卵泡刺激素(FSH)、促黃體生成素(LH)水平,同步測定乳痠脫氫酶(LDH)、γ-穀氨痠轉肽酶(γ-GT)、痠性燐痠酶(ACP)、葡萄糖-6-燐痠脫氫酶(G-6-PD)的活力.結果 與陰性對照組比較,1/8LD50 VOCs染毒組小鼠睪汍繫數明顯增高,差異有統計學意義(P＜0.05).各染毒組的睪汍標誌酶活力隨著染毒劑量的增加呈現下降趨勢,與陰性對照組比較,差異有統計學意義(P＜0.05).各染毒組的睪汍性激素水平隨著染毒劑量的增加呈現下降趨勢,與陰性對照組比較,差異有統計學意義(P＜0.05).結論 VOCs對雄性小鼠睪汍具有明顯的毒性,可影響其睪汍性激素水平和酶活力.
목적 연구다충휘발성유궤물(VOCs)대소서고환조직상관매급격소적영향.방법 급성독성시험학정VOCs적LD50후,선택청길급곤명충순계웅성소서40지,분위1/8 LD50 VOCs염독조、1/4 LD50VOCs염독조、1/2 LD50 VOCs염독조、배린선알(60 mg/kg)양성대조조화다유음성대조조,매조8지,련속복강주사5d,응용ELISA법측정소서고환조직중격소고동(T)、자이순(E2)、란포자격소(FSH)、촉황체생성소(LH)수평,동보측정유산탈경매(LDH)、γ-곡안산전태매(γ-GT)、산성린산매(ACP)、포도당-6-린산탈경매(G-6-PD)적활력.결과 여음성대조조비교,1/8LD50 VOCs염독조소서고환계수명현증고,차이유통계학의의(P＜0.05).각염독조적고환표지매활력수착염독제량적증가정현하강추세,여음성대조조비교,차이유통계학의의(P＜0.05).각염독조적고환성격소수평수착염독제량적증가정현하강추세,여음성대조조비교,차이유통계학의의(P＜0.05).결론 VOCs대웅성소서고환구유명현적독성,가영향기고환성격소수평화매활력.
Objective To evaluate the toxic effects of mixture of volatile organic compounds(VOCs) on Mice Testis related enzymes and hormones.Methods After determining the median lethal dose (LD50) of VOCs using the acute toxicity test,40 male clean inbred Kunming mice were assigned to 1/8 LD50 VOCs exposure group,1/4 LD50 VOCs exposure group,and 1/2 LD50 VOCs exposure group,as well as positive control group with cyclophosphamide (60 mg/kg) and negative control group with tea oil,with 8 mice in each group.The mice were intraperitoneally injected with respective agents for 5 days.The levels of testis testosterone,estradiol,follicle stimulating hormone,and luteinizing hormone were determined by ELISA.Meanwhile,the activity of testicular marked enzymes such as lactate dehydrogenase,gamma-glutamyl transpeptidase,acid phosphatase,and glucose-6-phosphate dehydrogenase were examined.Results Compared with the negative control group,the 1/8 LD50 exposure group had a significantly increased testis coefficient (P＜0.05).Both the activity of testicular marked enzymes and the levels of testicular sex hormones in all exposure groups showed significant downward trends with increasing VOC doses compared with those in the negative control group (P＜0.05).Conclusion VOCs have obvious toxicity to mouse testis by changing the levels of testicular sex hormones and the activity of testicular marked enzymes.
