CAJ | 학술논문

目的观察小鼠皮肤染毒火箭煤油对小鼠血象和血清中细胞因子的影响.方法 ICR小鼠背部剃毛,随机分为正常对照组和火箭煤油组,分别涂抹麻油和火箭煤油原油(400 μl×1次).染毒后2、6、12h和1、3、7d测定外周血中白细胞、红细胞、血红蛋白、血小板、淋巴细胞、单核细胞和中性粒细胞数量和染毒后2、6、12h和1、3、5、7d用流式细胞术的微阵列技术检测血清中白细胞介素(IL)-2、IL-4、IL-6、IL-10、肿瘤坏死因子-α(TNF-α)、干扰素(IFN)-γ和IL-17A的含量.结果与正常对照组比较,火箭煤油染毒组染毒后6、12h和1d白细胞和淋巴细胞数量明显下降,染毒后7d白细胞和淋巴细胞数量明显增多,差异均有统计学意义(P＜0.05).与正常对照组比较,火箭煤油组染毒后2、6、12h和1、3 d IL-6含量明显增高,染毒后2h和3、5、7 d IL-10的含量明显增高,差异均有统计学意义(P＜0.05);火箭煤油组染毒后2、6h和3、5、7 d TNF-α的含量明显增高,染毒后6h和1、3 d IFN-γ的含量明显增高,染毒后3、5、7 d IL-17A的含量明显增高,差异均有统计学意义(P＜0.05).结论皮肤染毒火箭煤油可以改变炎症细胞数量,引起机体细胞因子变化.
목적 관찰소서피부염독화전매유대소서혈상화혈청중세포인자적영향.방법 ICR소서배부체모,수궤분위정상대조조화화전매유조,분별도말마유화화전매유원유(400 μl×1차).염독후2、6、12h화1、3、7d측정외주혈중백세포、홍세포、혈홍단백、혈소판、림파세포、단핵세포화중성립세포수량화염독후2、6、12h화1、3、5、7d용류식세포술적미진렬기술검측혈청중백세포개소(IL)-2、IL-4、IL-6、IL-10、종류배사인자-α(TNF-α)、간우소(IFN)-γ화IL-17A적함량.결과 여정상대조조비교,화전매유염독조염독후6、12h화1d백세포화림파세포수량명현하강,염독후7d백세포화림파세포수량명현증다,차이균유통계학의의(P＜0.05).여정상대조조비교,화전매유조염독후2、6、12h화1、3 d IL-6함량명현증고,염독후2h화3、5、7 d IL-10적함량명현증고,차이균유통계학의의(P＜0.05);화전매유조염독후2、6h화3、5、7 d TNF-α적함량명현증고,염독후6h화1、3 d IFN-γ적함량명현증고,염독후3、5、7 d IL-17A적함량명현증고,차이균유통계학의의(P＜0.05).결론 피부염독화전매유가이개변염증세포수량,인기궤체세포인자변화.
Objective To detect the number of cells and the level of IL-2,IL-4,IL-6,IL-10,TNF-alpha,IFN-γ and IL-17 cytokines in the peripheral blood of mice exposed to rocket kerosene by skin.Method [CR mice were randomly divided into the normal control group and RK experimental group (400 μl×1 group).RK undiluted fuel were applied directly to the dorsal skin of the mice.In control groups were treated with sesame oil (SO).the number of blood cells were detected by automatic blood cell counter and the level of IL-2,IL-4,IL-6,IL-10,TNF-alpha,IFN-γ and IL-17 cytokines in serum were detected by using flow cytometry and BD CBA Flex set kit.Result Compared with the normal group,WBC and LYM had a decreasing tendency 2 h and decreased significantly 6 h,12 h and 1 d after RK exposure (P＜0.05).They increased significantly 7 d after RK exposure (P＜0.05).Compared with the normal group,the level of IL-6 increased significantly 2 h,6 h,12 h,1 d and 3 d (P＜0.05).The level of TNF-α increased significantly 2h,3d,5d and 7d (P＜0.05).The level of IL-10 increased significantly 2 h,6 h,3 d,5 d and 7 d (P＜0.05).The level of IFN-γ increased significantly 6 h and 3 d (P＜ 0.05).The level of IL-17 significantly increased 3 d,5 d and 7d (P＜0.05).Conclusion RK can change the number of immune cells,causing the immune cytokine changes in mice after RK cutaneous exposure.