CAJ | 학술논문

目的：研究桑枝降血糖的活性部位，对桑枝进行提取分离，初步得出降糖活性成分。方法桑枝渗漉后经过不同溶剂萃取，通过小鼠体内降糖实验，以高糖小鼠的血糖下降百分率为指标，确定桑枝的降糖活性萃取部位。采用聚酰胺树脂对活性萃取部位进行分离，同样通过降糖实验，以高糖小鼠的血糖下降百分率为指标，确定桑枝的降糖活性分离部位。采用理化显色反应，初步鉴别降糖活性物质。结果通过对桑枝提取液萃取后，降糖效果最明显的为正丁醇萃取部位，高糖小鼠血糖下降百分率为26.20%。正丁醇萃取部位采用聚酰胺树脂分离后降糖效果最明显的为10%乙醇洗脱部位，血糖下降百分率为31%。理化显色反应结果表明活性部位中含有生物碱类物质。结论初步确定桑枝中降血糖活性部位中存在生物碱类成分。
목적：연구상지강혈당적활성부위，대상지진행제취분리，초보득출강당활성성분。방법상지삼록후경과불동용제췌취，통과소서체내강당실험，이고당소서적혈당하강백분솔위지표，학정상지적강당활성췌취부위。채용취선알수지대활성췌취부위진행분리，동양통과강당실험，이고당소서적혈당하강백분솔위지표，학정상지적강당활성분리부위。채용이화현색반응，초보감별강당활성물질。결과통과대상지제취액췌취후，강당효과최명현적위정정순췌취부위，고당소서혈당하강백분솔위26.20%。정정순췌취부위채용취선알수지분리후강당효과최명현적위10%을순세탈부위，혈당하강백분솔위31%。이화현색반응결과표명활성부위중함유생물감류물질。결론초보학정상지중강혈당활성부위중존재생물감류성분。
Objective To study the active part of Ramulus Mori that reduces blood glucose,so as to extract it and obtain the active parts. Methods The percentage of reduction blood glucose was taken as in-dex and the in vivo hypoglycemic experiment was used to optimize the active components which can lower the blood glucose percolated from Ramulus Mori extracting solution. Polyamide was used to separate the active ex-traction,and the percentage of reduction blood glucose was also taken as index and the same experiment was used to optimize the active separated components which could lower the blood. Chromogenic reaction was used to identify the active extraction preliminarily. Results The experiment proved that the most effective extrac-tion was Ramulus Mori extracted by n - butyl alcohol and the percentage of reducing blood glucose was 26. 20% . The active extraction was separated by polyamide used different concentration of ethanol,then the most effective part was eluted by 10% ethanol and the percentage of reducing blood glucose was 31% . The result of chromogenic reaction proved that alkaloid compounds and amino acids were existed in the effective part. Conclusion It is initially identified that alkaloid compounds exist in active sites of reducing blood glu-cose in Ramulus Mori.