CAJ | 학술논문

目的：探讨复方片仔癀肝宝（GB）对急性肝损伤的保护作用。方法将60只 SD 大鼠随机分为正常组、模型组、西利宾胺组（阳性对照）、GB 低剂量组、GB 中剂量组、GB 高剂量组，每组10只。连续给药7 d 后，采用 CCl4诱导急性肝损伤模型，24 h 后检测 GB 对 ALT、AST、ALP 的影响；HE染色观察 GB 对肝组织病理学的影响；Elisa 法检测 GB 对 TNF -α和 IL -1β的影响。结果各实验组 ALT、AST、ALP 的活性显著降低，观察肝脏病理切片各实验组与模型组比较，肝损伤均有减轻的趋势；血清中 TNF -α和 IL -1β的含量降低，差异有统计学意义（P ﹤0.05）。结论 GB 能显著减轻CCl4诱导的急性肝损伤，可能与 GB 的抗炎症反应有关。
목적：탐토복방편자황간보（GB）대급성간손상적보호작용。방법장60지 SD 대서수궤분위정상조、모형조、서리빈알조（양성대조）、GB 저제량조、GB 중제량조、GB 고제량조，매조10지。련속급약7 d 후，채용 CCl4유도급성간손상모형，24 h 후검측 GB 대 ALT、AST、ALP 적영향；HE염색관찰 GB 대간조직병이학적영향；Elisa 법검측 GB 대 TNF -α화 IL -1β적영향。결과각실험조 ALT、AST、ALP 적활성현저강저，관찰간장병리절편각실험조여모형조비교，간손상균유감경적추세；혈청중 TNF -α화 IL -1β적함량강저，차이유통계학의의（P ﹤0.05）。결론 GB 능현저감경CCl4유도적급성간손상，가능여 GB 적항염증반응유관。
Objective To explore the protective action of complex prescription Pianzaihuang Ganbao on acute liver injury. Methods Sixty Sprague Dawley rats were divided into six groups randomly as follows:control group,the model group,Silymarin group(positive control),Ganbao(GB)low dose group,GB middle dose group,GB high dose group. The rats were injected carbon tetrachloride(CCl4 )subcutaneously to estab-lish the acute liver injury model,except for the rats in control group. Rats in therapeutic groups were pretrea-ted with GB and silymarin seven days before CCl4 was given. After 24 hours,serum was obtained to determine the activity of ALT、AST and ALP. Hematoxylin - Eosin( HE)staining was used to detect the pathological change. Enzyme linked immunosorbent assay(Elisa)was used to explore the expression of TNF - αand IL -1β. Results GB and silymarin could inhibit the activity of ALT,AST and ALP. Both GB and silymarin could ameliorate the pathology of liver and decrease the expression of TNF - αand IL - 1β. Conclusion GB could relieve the liver injury induced by CCl4 ,maybe correlate with its anti - inflammation activity.