中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
Chinese Journal of Industrial Hygiene and Occupational Diseases
2015年
9期
641-645
,共5页
劳灿山%张迎建%李文超%李明月%杨红
勞燦山%張迎建%李文超%李明月%楊紅
로찬산%장영건%리문초%리명월%양홍
二氯化硅%miR-144%肺损伤%肺纤维化
二氯化硅%miR-144%肺損傷%肺纖維化
이록화규%miR-144%폐손상%폐섬유화
Silicon dioxide%miR-144%Pulmonary injury%Pulmonary fibrosis
目的 探索miR-144在纳米二氧化硅(SiO2)致大鼠肺损伤中的生物学作用.方法 150只健康雄性SD大鼠随机分生理盐水对照组和6.25、12.5、25.0 mg/ml纳米SiO2组及25.0 mg/ml微米SiO2组,每组30只,分别于染尘7、15、30、60、90 d后各处死6只,观察各时间点大鼠肺组织的病理改变.用实时荧光定量聚合酶链式反应(qRT-PCR)法检测大鼠经气管一次性灌注25 mg/ml纳米SiO290 d后肺组织中miR-144的表达水平;用Targetscan、microRNA.org及miRDB 3个数据库对miR-144进行靶基因预测;用the Gene Ontology、KEGG分别对预测靶基因进行功能显著性富集分析和信号通路分析,筛选与肺纤维化相关的靶基因.结果 25.0 mg/ml纳米SiO2染尘90 d组大鼠肺组织中miR-144表达上调,与Hiseq 2000高通量测序的结果一致;筛选miR-144的预测靶基因为SMAD4、SMAD5、ADAMTS3、ADAMTS15、ADAMTS19等,这些基因可能与纤维化相关或参与纤维化通路.结论 miR-144可能参与肺纤维化的调控,在纳米SiO2所致肺损伤中发挥一定作用.
目的 探索miR-144在納米二氧化硅(SiO2)緻大鼠肺損傷中的生物學作用.方法 150隻健康雄性SD大鼠隨機分生理鹽水對照組和6.25、12.5、25.0 mg/ml納米SiO2組及25.0 mg/ml微米SiO2組,每組30隻,分彆于染塵7、15、30、60、90 d後各處死6隻,觀察各時間點大鼠肺組織的病理改變.用實時熒光定量聚閤酶鏈式反應(qRT-PCR)法檢測大鼠經氣管一次性灌註25 mg/ml納米SiO290 d後肺組織中miR-144的錶達水平;用Targetscan、microRNA.org及miRDB 3箇數據庫對miR-144進行靶基因預測;用the Gene Ontology、KEGG分彆對預測靶基因進行功能顯著性富集分析和信號通路分析,篩選與肺纖維化相關的靶基因.結果 25.0 mg/ml納米SiO2染塵90 d組大鼠肺組織中miR-144錶達上調,與Hiseq 2000高通量測序的結果一緻;篩選miR-144的預測靶基因為SMAD4、SMAD5、ADAMTS3、ADAMTS15、ADAMTS19等,這些基因可能與纖維化相關或參與纖維化通路.結論 miR-144可能參與肺纖維化的調控,在納米SiO2所緻肺損傷中髮揮一定作用.
목적 탐색miR-144재납미이양화규(SiO2)치대서폐손상중적생물학작용.방법 150지건강웅성SD대서수궤분생리염수대조조화6.25、12.5、25.0 mg/ml납미SiO2조급25.0 mg/ml미미SiO2조,매조30지,분별우염진7、15、30、60、90 d후각처사6지,관찰각시간점대서폐조직적병리개변.용실시형광정량취합매련식반응(qRT-PCR)법검측대서경기관일차성관주25 mg/ml납미SiO290 d후폐조직중miR-144적표체수평;용Targetscan、microRNA.org급miRDB 3개수거고대miR-144진행파기인예측;용the Gene Ontology、KEGG분별대예측파기인진행공능현저성부집분석화신호통로분석,사선여폐섬유화상관적파기인.결과 25.0 mg/ml납미SiO2염진90 d조대서폐조직중miR-144표체상조,여Hiseq 2000고통량측서적결과일치;사선miR-144적예측파기인위SMAD4、SMAD5、ADAMTS3、ADAMTS15、ADAMTS19등,저사기인가능여섬유화상관혹삼여섬유화통로.결론 miR-144가능삼여폐섬유화적조공,재납미SiO2소치폐손상중발휘일정작용.
Objective To Investigate the biological effects of miR-144 in rats' pulmanory injury induced by nanosized SiO2 preliminarily.Method 150 healthy SD rats were divided into five groups randomly:the control group,the nanosized SiO2 groups of 6.25,12.5,25.0 mg/ml,and the microsized SiO2 group of 25.0 mg/ml,30 rats each group.Six rats were sacrificed for their pathological change on the 7th,15th,30th,60th and 90th day after exposure.The expression levels of mature miR-144 in lung tissue of the rats after instilled intracheally nanosized SiO2 at 90d was detected by Quantitative Reverse Transcription PCR.Target prediction for miR-144 was conducted by databases of Target-scan,microRNA.org and miRDB.Function-significant enrichment analysis and signal pathway analysis for predicted target genes were respectively conducted by the Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes,then target genes related to pulmonary fibrosis were screened out.Results The expression of miR-144 was up-regulated in lung tissue of rats exposed to nanosized SiO2.The result was consistent with the results of high-throughput sequencing Hiseq 2000.The target genes of miR-144 related to fibrosis or signal pathway involved in fibrosis were screened out.They are SMAD4,SMAD5,ADAMTS3,ADAMTS15 and ADAMTS19.Conclusion MiR-144 probably participate in the regulation of fibrosis,which may play an important role in pulmonary injury induced by nanosized SiO2.
