CAJ | 학술논문

基于异丙肾上腺素（IP）对 luminol‐KIO4化学发光体系信号的增强作用，发展了一种流动注射化学发光灵敏检测异丙肾上腺素的新方法．对影响化学发光强度和重现性的参数进行了系统优化．在优化条件下，该方法对 IP 检测的线性范围为5．0×10－9～1．5×10－6 mol／L ，检出限为5．8×10－10 mol／L ．对5．0×10－7 mol／L 的 IP 溶液平行测定12次的相对标准偏差为1．7％，分析通量为130个／h ．将该方法应用于药物制剂盐酸异丙肾上腺素注射液分析，加标回收率为99．2％～103．2％．利用化学发光光谱和紫外光谱对该化学发光反应的增强机理进行了探讨．
기우이병신상선소（IP）대 luminol‐KIO4화학발광체계신호적증강작용，발전료일충류동주사화학발광령민검측이병신상선소적신방법．대영향화학발광강도화중현성적삼수진행료계통우화．재우화조건하，해방법대 IP 검측적선성범위위5．0×10－9～1．5×10－6 mol／L ，검출한위5．8×10－10 mol／L ．대5．0×10－7 mol／L 적 IP 용액평행측정12차적상대표준편차위1．7％，분석통량위130개／h ．장해방법응용우약물제제염산이병신상선소주사액분석，가표회수솔위99．2％～103．2％．이용화학발광광보화자외광보대해화학발광반응적증강궤리진행료탐토．
A facile and sensitive method for determination of isoprenaline was developed by flow injection coupled with chemiluminescence detection based on significant signal enhancement of isoprenaline on luminol‐KIO4 system .Parameters affecting both chemiluminescence intensity and reproducibility were optimized .Under the optimum conditions ,the method psseses linear range from 5 .0 × 10 - 9 to 1 .5 × 10 - 6 mol/L with detection limit of 5 .8 × 10 - 10 mol/L .The relative standard deviation was found to be 1 .7% for 12 replicate determinations of 5.0 × 10 - 7 mol/L isoprenaline .The analyzing throughput of the proposed method is about 130 samples/h . The application of the present method was demonstrated by analyzing isoprenaline in isoprenaline hydrochloride injection with the recoveries of 99 .2% ~ 103 .2% .The possible mechanism of the enhanced chemiluminescence intensity was discussed by chemiluminescence spectra and UV‐vis spectra .