生物学杂志
生物學雜誌
생물학잡지
Journal of Biology
2015年
5期
63-66,69
,共5页
李威%黄进%李其昌%苏宝连%谢浩
李威%黃進%李其昌%囌寶連%謝浩
리위%황진%리기창%소보련%사호
纳米颗粒%叶绿体%光合活性%光合作用
納米顆粒%葉綠體%光閤活性%光閤作用
납미과립%협록체%광합활성%광합작용
nanoparticles%chloroplast%photosynthetic activity%photosynthesis
为探讨纳米颗粒对植物光合作用的影响,采取一种简单、高效的实验方法来进行研究。选取具有代表性的5种金属氧化物纳米颗粒与植物光合作用的主要场所叶绿体相互作用,测定叶绿体光合活性、荧光效应的变化,并通过扫描电镜、荧光显微镜进行观察。研究表明纳米颗粒对叶绿体光合活性的影响是通过相互接触完成的;ZnO及CdS对其活性有明显的抑制效应,并随着浓度的增加其抑制效应越显著;γ-Fe2 O3纳米颗粒作为电子受体,在低浓度条件下提高其活性,但随着浓度的增加逐渐显现为抑制效应。故而推测纳米颗粒的影响机制是影响电子传递过程。这为纳米材料在植物领域,包括纳米化肥,纳米仿生,可再生性高效生化探测器的发展提供了参考,同时也为评估纳米材料对环境的潜在影响提供了理论基础。
為探討納米顆粒對植物光閤作用的影響,採取一種簡單、高效的實驗方法來進行研究。選取具有代錶性的5種金屬氧化物納米顆粒與植物光閤作用的主要場所葉綠體相互作用,測定葉綠體光閤活性、熒光效應的變化,併通過掃描電鏡、熒光顯微鏡進行觀察。研究錶明納米顆粒對葉綠體光閤活性的影響是通過相互接觸完成的;ZnO及CdS對其活性有明顯的抑製效應,併隨著濃度的增加其抑製效應越顯著;γ-Fe2 O3納米顆粒作為電子受體,在低濃度條件下提高其活性,但隨著濃度的增加逐漸顯現為抑製效應。故而推測納米顆粒的影響機製是影響電子傳遞過程。這為納米材料在植物領域,包括納米化肥,納米倣生,可再生性高效生化探測器的髮展提供瞭參攷,同時也為評估納米材料對環境的潛在影響提供瞭理論基礎。
위탐토납미과립대식물광합작용적영향,채취일충간단、고효적실험방법래진행연구。선취구유대표성적5충금속양화물납미과립여식물광합작용적주요장소협록체상호작용,측정협록체광합활성、형광효응적변화,병통과소묘전경、형광현미경진행관찰。연구표명납미과립대협록체광합활성적영향시통과상호접촉완성적;ZnO급CdS대기활성유명현적억제효응,병수착농도적증가기억제효응월현저;γ-Fe2 O3납미과립작위전자수체,재저농도조건하제고기활성,단수착농도적증가축점현현위억제효응。고이추측납미과립적영향궤제시영향전자전체과정。저위납미재료재식물영역,포괄납미화비,납미방생,가재생성고효생화탐측기적발전제공료삼고,동시야위평고납미재료대배경적잠재영향제공료이론기출。
To investigate the effect of nanoparticles ( NPs) on plant photosynthesis, a simple and efficient approach was adopted.Five typical representative mental oxide NPs were chosen to interacted with the chloroplast, the reaction center of photosynthesis of plant. The variation of photosynthetic activity and fluorescence effect were measured.Scanning electron microscopy ( SEM) and fluorescent microscopy showed that the impact of NPs on the photosynthetic activity of chloroplast was achieved by contacting with each other.The results indicated that ZnO and CdS NPs had obvious inhibiting effect, and with the increase of concentration the inhibition was more significantly;γ-Fe2 O3 NPs can be used as electron acceptor, had some promoting effect at low concentration, but with the increase of the concentration the inhibition effect appeared gradually.Suggesting the impact mechanism of NPs was affecting the process of the e-lectron transfer.This work provides a promising approach for the development of the field of NPs in plants, including nano-fertilizer, nanobionics and a renewable, high-efficiency biochemical detectors.Meanwhile, it provides a theoretical basis for evaluating the poten-tial effects of NPs on environment.