CAJ | 학술논문

目的：建立一种高效液相色谱法（ high performance liquid chromatography，HPLC），用来测定人头发中的喹硫平浓度。方法色谱柱使用Inertsil ODS-C18柱（4.6 mm ×150 mm，5μm），流动相为0.01 mol／L醋酸铵-甲醇（32︰68），流速为1.0 mL／min，柱温40℃，检测波长为254 nm，以正已烷为萃取剂，建立HPLC法。结果喹硫平在5～200 ng／mg范围内峰面积与浓度呈良好线性关系，萃取回收率＞75.0％，方法回收率在95.0％～105.0％之间，日内和日间精密度均＜10.0％，符合生物样品分析要求。结论本研究建立了测定人头发中喹硫平浓度的HPLC法，该方法操作简单，结果灵敏、准确，具有一定的应用价值。
목적：건립일충고효액상색보법（ high performance liquid chromatography，HPLC），용래측정인두발중적규류평농도。방법색보주사용Inertsil ODS-C18주（4.6 mm ×150 mm，5μm），류동상위0.01 mol／L작산안-갑순（32︰68），류속위1.0 mL／min，주온40℃，검측파장위254 nm，이정이완위췌취제，건립HPLC법。결과규류평재5～200 ng／mg범위내봉면적여농도정량호선성관계，췌취회수솔＞75.0％，방법회수솔재95.0％～105.0％지간，일내화일간정밀도균＜10.0％，부합생물양품분석요구。결론본연구건립료측정인두발중규류평농도적HPLC법，해방법조작간단，결과령민、준학，구유일정적응용개치。
Objective To establish a method for determinning quetiapine in human hair by HPLC.Methods A reverse phase HPLC system was performed on Inertsil ODS-C18 column (4.6 mm ×150 mm,5μm)with the mobile phase consisted of 0.01 mol/Lammonioum-methanol(32︰68).The flow rate was 1.0 mL/min,column temperature was 40℃,the detection wavelength was 254 nm.N-hexane was used as extracting solvent.Results The calibration curve was linear in the range of 5-200 ng/mg.for quetiapine.The recovery of extraction >75.0%, the recovery of method was between 95.0% and 105.0%, the intra-day and inter-day precision were all no more than 10.0%.This method met the requirements of biological samples. Conclusion A HPLC method of concentration of quetiapine in human hair is established, which is simple,sensitive,accurate and has a certain value.