CAJ | 학술논문

以Cortland液为稀释液、10％EG为抗冻剂、0．25 mL麦细管为冻存管，两步降温法超低温冷冻中国花鲈精子，对冷冻前后精子活力及6种酶活性变化进行了测定。结果表明，冻存15 d与30 d的冻精激活率及运动时间分别为（86．67±3．79）％及（15．08±0．72） min、（86．00±3．61）％及（14．83±1．23） min，与鲜精激活率（92．67±2．52）％及运动时间（15．75±1．25）min相比无显著差异。冻存15 d与30 d的冻精总ATP酶、SDH、LDH、SOD、CAT和GR活性分别为：（73．09±3．94） U／mL与（71．57±5．51） U／mL、（44．67±4．93） U／mL与（43．67±4．93） U／mL、（6916．93±265．45）U／L与（6889．73±262．63）U／L、（84．80±4．08）U／mL与（84．42±3．09）U／mL、（92．37±7．64） U ／mL与（92．13±7．37）U／mL、（79．01±3．93）U／L与（75．91±2．95）U／L，与鲜精酶活性相比差异不显著。认为以Cortland液为稀释液、10％EG为抗冻剂、两步降温法超低温冷冻中国花鲈精子的效果较好。
이Cortland액위희석액、10％EG위항동제、0．25 mL맥세관위동존관，량보강온법초저온냉동중국화로정자，대냉동전후정자활력급6충매활성변화진행료측정。결과표명，동존15 d여30 d적동정격활솔급운동시간분별위（86．67±3．79）％급（15．08±0．72） min、（86．00±3．61）％급（14．83±1．23） min，여선정격활솔（92．67±2．52）％급운동시간（15．75±1．25）min상비무현저차이。동존15 d여30 d적동정총ATP매、SDH、LDH、SOD、CAT화GR활성분별위：（73．09±3．94） U／mL여（71．57±5．51） U／mL、（44．67±4．93） U／mL여（43．67±4．93） U／mL、（6916．93±265．45）U／L여（6889．73±262．63）U／L、（84．80±4．08）U／mL여（84．42±3．09）U／mL、（92．37±7．64） U ／mL여（92．13±7．37）U／mL、（79．01±3．93）U／L여（75．91±2．95）U／L，여선정매활성상비차이불현저。인위이Cortland액위희석액、10％EG위항동제、량보강온법초저온냉동중국화로정자적효과교호。
In order to investigate damage mechanism of sperm after cryopreservation(-196℃) , we used 10%EG( ethyl-ene glycol) as cryoprotectant, Cortland as extender, and two-step cooling procedure to cryopreserve Lateolabrax maculatus sperm in 0.25 mL straws.The motility of fresh sperm and frozen-thawed sperm was detected by microscopy and the en-zyme activities by reagent kits.The results showed that there were no significant differences between fresh sperm and fro-zen-thawed sperm of freezing for 15 d or 30 d in motility.The activation rate, moving time and life-span of fresh sperm were (92.67 ±2.52)%, (15.75 ±1.25)min and (20.67 ±1.76)min, and (86.67 ±3.79)%, (15.08 ±0.72)min and (19.83 ±1.44)min of frozen-thawed sperm for freezing 15 d, and (86.00 ±3.61)%, (14.83 ±1.23)min and (19.67 ±0.52)min of freezing for 30 d.The activities of total ATPase, SDH, LDH, SOD, CAT and GR of frozen-thawed sperm after cryopreservation for 15 d and 30 were (73.09 ±3.94)U/mL, (44.67 ±4.93)U/mL, (6916.93 ±265.45) U/L, (84.80 ±4.08) U/mL, ( 92.37 ±7.64 ) U/mL, ( 79.01 ±3.93 ) U/L and ( 71.57 ±5.51 ) U/mL, ( 43.67 ± 4.93)U/mL, (6889.73 ±262.63)U/L, (84.42 ±3.09)U/mL, (92.13 ±7.37)U/mL, (75.91 ±2.95)U/L, respec-tively.In addition to GR, the other enzyme activities all decreased with the freezing time prolonged.Enzyme activities de-tection showed that there was no significant difference between fresh sperm and frozen-thawed sperm in Lateolabrax macu-latus.So we concluded that cryopreservation had no significant effects on the enzyme activities and motility of sperm in Lateolabrax maculatus using the previous procedure.