CAJ | 학술논문

利用RACE技术从蚤状溞（ Daphnia pluex）中克隆到doublesex（ dpdsx），dpdsx cDNA全长为1636 bp，其中开放阅读框（ ORF）1008 bp，编码335个氨基酸，含有1个DM结构域和1个Dimersation domain。同源性比对结果显示蚤状溞dpdsx与大型溞（ Daphnia magna）同源性最高为77％，其次盔形溞（ Daphnia galeata）为61％，模糊网纹溞（ Ce－riodaphnia dubia）为54％；进化分析发现蚤状溞dpdsx与盔形溞、大型溞、模糊网纹溞等溞科的亲缘关系最近；Real time－PCR实验结果表明，dpdsx在雄溞表达水平最高，其次是孤雌溞（带夏卵）和两性溞（带冬卵），雌溞与雄溞有显著性差异（ P＞0．05）。表明doublesex有可能在蚤状溞生殖转化和性别分化调控上起一定作用。
이용RACE기술종조상소（ Daphnia pluex）중극륭도doublesex（ dpdsx），dpdsx cDNA전장위1636 bp，기중개방열독광（ ORF）1008 bp，편마335개안기산，함유1개DM결구역화1개Dimersation domain。동원성비대결과현시조상소dpdsx여대형소（ Daphnia magna）동원성최고위77％，기차회형소（ Daphnia galeata）위61％，모호망문소（ Ce－riodaphnia dubia）위54％；진화분석발현조상소dpdsx여회형소、대형소、모호망문소등소과적친연관계최근；Real time－PCR실험결과표명，dpdsx재웅소표체수평최고，기차시고자소（대하란）화량성소（대동란），자소여웅소유현저성차이（ P＞0．05）。표명doublesex유가능재조상소생식전화화성별분화조공상기일정작용。
The full-length cDNA of a doublesex ( dpdsx) gene was cloned from the cladoceran Daphnia pulex using rapid amplification of complementary DNA ends ( RACE ) method.The dpdsx cDNA is 1636 bp in length; and it has a 1008-bp open reading frame ( ORF) that encodes a 335-amino-acid polypeptide containing a DM structural domain and a dimersation domain.Homology comparison showed Daphnia pulex′s dpdsx sharing homology of up to 77%with Daphnia magna, 61% with Daphnia magna and 54% with Ceri-odaphnia dubia.Phylogenetic analysis revealed that Daphnia pulex′s dpdsx had a close genetic relationship with Daphniidae such as D. magna, D.magna, C.dubia and so on.Real time-PCR showed that dpdsx had the highest expression level in the male, followed by the parthenogenetic female ( with summer egg) and sexual female ( with winter egg) .The expression had significant difference ( P>0.05) between the male and the female.The experiment indicated that doublesex gene may be play a role in reproduction switching and controlling sex differentiation of D.pulex.