CAJ | 학술논문

目的：分析上调基因－4（URG4）在宫颈癌中的表达，并观察分析抑制URG4对宫颈癌的治疗作用及机制。方法：Western blot 法分析URG4在宫颈癌组织及癌旁组织中的表达差异。选择16只雄性BALB／c 裸鼠，喂养至6周龄后随机分为对照组（Control 组）及观察组（Observation 组），每组各8只。Control 组小鼠于右侧背部皮下接种宫颈癌细胞（SiHa cells）。Observation 组小鼠背部皮下接种URG4－siRNA 转染的宫颈癌细胞（URG4－siRNA SiHa cells）。干预后4周处死小鼠，分析对比小鼠肿瘤生长情况及Wnt／β－catenin 信号通路的改变。结果：URG4在人宫颈癌组织中呈现高表达，显著高于癌旁组织（P＜0．05）。干预结束后，观察组小鼠肿瘤组织内URG4表达量显著低于对照组（P＜0．05）；体重明显高于对照组（P＜0．05）；肿瘤组织重量显著低于对照组（P＜0．05）。观察组小鼠肿瘤组织内Ki67表达量显著低于对照组（P＜0．05）。观察组小鼠肿瘤组织内CyclinD1、β－catenin 及C－myc 的表达均显著低于对照组，相比较差异均具有统计学意义（P＜0．05）。结论：URG4在人宫颈癌中呈现高表达，抑制URG4的表达可能通过抑制Wnt／β－catenin 信号通路而发挥抗肿瘤效应。
목적：분석상조기인－4（URG4）재궁경암중적표체，병관찰분석억제URG4대궁경암적치료작용급궤제。방법：Western blot 법분석URG4재궁경암조직급암방조직중적표체차이。선택16지웅성BALB／c 라서，위양지6주령후수궤분위대조조（Control 조）급관찰조（Observation 조），매조각8지。Control 조소서우우측배부피하접충궁경암세포（SiHa cells）。Observation 조소서배부피하접충URG4－siRNA 전염적궁경암세포（URG4－siRNA SiHa cells）。간예후4주처사소서，분석대비소서종류생장정황급Wnt／β－catenin 신호통로적개변。결과：URG4재인궁경암조직중정현고표체，현저고우암방조직（P＜0．05）。간예결속후，관찰조소서종류조직내URG4표체량현저저우대조조（P＜0．05）；체중명현고우대조조（P＜0．05）；종류조직중량현저저우대조조（P＜0．05）。관찰조소서종류조직내Ki67표체량현저저우대조조（P＜0．05）。관찰조소서종류조직내CyclinD1、β－catenin 급C－myc 적표체균현저저우대조조，상비교차이균구유통계학의의（P＜0．05）。결론：URG4재인궁경암중정현고표체，억제URG4적표체가능통과억제Wnt／β－catenin 신호통로이발휘항종류효응。
Objective:To analyze the curative effect of suppression expression of URG4 on cervical cancer mice and the possible mechanisms.Methods:The expression of URG4 were analyzed in cervical cancer tissues and para-carcinoma tissues.16 male BALB/c nude mice were divided into Group A ( inoculated with URG4-siRNA SiHa cells) and Group B ( inoculated with SiHa cells) . After 4 weeks,mice were executed and analyzed.Results:The expression of URG4 were higher in human cervical cancer tissues than that in para-carcinoma tissues ( P<0.05).After experiment,Group A had a lower tumor weight,but a higher body weight than these of Group B,the difference was statistically significant (P<0.05).Group A mice had a lower levels of URG4 in tumor than that of Group B mice (P<0.05).Group A mice had a lower levels of Ki67 in tumor than that of Group B mice,the difference was statistically significant (P<0.05).Group A mice had lower levels of CyclinD1,β-catenin and C-myc in tumor than these of Group B mice (P<0.05). Conclusion:The expression of URG4 in cervical cancer tissue is up-regulation.Suppression expression of URG4 can suppress tumor growth in vivo,which may though suppress the Wnt/β-catenin signaling pathway.