CAJ | 학술논문

目的：研究4℃条件下存放30 d后血浆中人巨细胞病毒DNA的稳定性。方法选择进行造血干细胞移植的150例患者的血浆标本，将其按照人巨细胞病毒DNA 载量分为3组：低载量组（n＝50，人巨细胞病毒DNA载量为300～1000 copy／mL）、中载量组（n＝50，人巨细胞病毒DNA载量为＞1000～10000 copy／mL）和高载量组（n＝50，人巨细胞病毒DNA载量为＞10000～100000 copy／mL）。标本采集后2 h内完成基线载量（0 d）检测，其余血浆在4℃条件下保存，分别在保存1、2、3、7、14、21、30 d时检测人巨细胞病毒载量。结果低、中、高载量组标本在保存1、2、3、7、14、21、30 d后，其人巨细胞病毒载量与0 d结果比较，差异均无统计学意义（ P＞0．05）。结论血浆标本在4℃条件下存放30 d对人巨细胞病毒载量检测结果无影响。
목적：연구4℃조건하존방30 d후혈장중인거세포병독DNA적은정성。방법선택진행조혈간세포이식적150례환자적혈장표본，장기안조인거세포병독DNA 재량분위3조：저재량조（n＝50，인거세포병독DNA재량위300～1000 copy／mL）、중재량조（n＝50，인거세포병독DNA재량위＞1000～10000 copy／mL）화고재량조（n＝50，인거세포병독DNA재량위＞10000～100000 copy／mL）。표본채집후2 h내완성기선재량（0 d）검측，기여혈장재4℃조건하보존，분별재보존1、2、3、7、14、21、30 d시검측인거세포병독재량。결과저、중、고재량조표본재보존1、2、3、7、14、21、30 d후，기인거세포병독재량여0 d결과비교，차이균무통계학의의（ P＞0．05）。결론혈장표본재4℃조건하존방30 d대인거세포병독재량검측결과무영향。
Objective To study the stability of cytomegalovirus (CMV) DNA in plasma after 30 d storage un‐der 4 ℃ .Methods The plasma samples from 150 patients undergoing hematopoietic stem cell transplantation were selected and divided into 3 groups according to DNA loads of human CMV :low load group(n=50 ,CMV DNA loads 300-1 000 copy/mL) ,middle load group(n=50 ,CMV DNA load 1 000 -10 000 copy/mL) and high loads group (n=50 ,CMV DNA loads 10 000-100 000 copy/mL) .The base line load(0 d) detection was completed within 2 d after sample collection ,the other palasma was stored under the condition of 4 ℃ .The human CMV load was detected on 1 ,2 ,3 ,7 ,14 ,30 d storage .Results The 150 samples were matched for different time points ,cytomegalovirus loads of 1 ,2 ,3 ,7 ,14 ,21 ,30 days compared with baseline ,the difference had no statistical significance (P>0 .05) .Conclu‐sion The plasma sample after 30 d storage under 4 ℃ has no influence on the detection results of CMV load .