交通医学
交通醫學
교통의학
Medical Journal of Communications
2015年
4期
329-332
,共4页
CDX2%DNA甲基化%胃癌
CDX2%DNA甲基化%胃癌
CDX2%DNA갑기화%위암
CDX2%DNA methylation%gastric neoplasm
目的：探讨胃癌患者癌组织及其外周血中CDX2基因启动子CpG岛甲基化的关系及其临床意义。方法：收集40例胃癌患者外周血和术后胃癌组织标本、12例胃良性病变旁正常胃组织以及20例健康体检者外周血标本，应用甲基化特异性PCR（MSP）检测组织及外周血中CDX2基因启动子CpG岛甲基化状态。结果：胃癌组织中CDX2基因启动子CpG岛甲基化率为72.5%，外周血中CDX2基因启动子CpG岛甲基化率为62.5%，差异无统计学意义（P>0.05）；胃癌组织及外周血中的CDX2基因启动子CpG岛甲基化呈正相关（r=0.5913，P=0.0012）。结论：胃癌组织及外周血中CDX2基因启动子CpG岛甲基化水平一致，检测患者外周血CDX2基因启动子CpG岛甲基化可为胃癌的临床诊治提供了一个简便、快捷的途径。
目的：探討胃癌患者癌組織及其外週血中CDX2基因啟動子CpG島甲基化的關繫及其臨床意義。方法：收集40例胃癌患者外週血和術後胃癌組織標本、12例胃良性病變徬正常胃組織以及20例健康體檢者外週血標本，應用甲基化特異性PCR（MSP）檢測組織及外週血中CDX2基因啟動子CpG島甲基化狀態。結果：胃癌組織中CDX2基因啟動子CpG島甲基化率為72.5%，外週血中CDX2基因啟動子CpG島甲基化率為62.5%，差異無統計學意義（P>0.05）；胃癌組織及外週血中的CDX2基因啟動子CpG島甲基化呈正相關（r=0.5913，P=0.0012）。結論：胃癌組織及外週血中CDX2基因啟動子CpG島甲基化水平一緻，檢測患者外週血CDX2基因啟動子CpG島甲基化可為胃癌的臨床診治提供瞭一箇簡便、快捷的途徑。
목적：탐토위암환자암조직급기외주혈중CDX2기인계동자CpG도갑기화적관계급기림상의의。방법：수집40례위암환자외주혈화술후위암조직표본、12례위량성병변방정상위조직이급20례건강체검자외주혈표본，응용갑기화특이성PCR（MSP）검측조직급외주혈중CDX2기인계동자CpG도갑기화상태。결과：위암조직중CDX2기인계동자CpG도갑기화솔위72.5%，외주혈중CDX2기인계동자CpG도갑기화솔위62.5%，차이무통계학의의（P>0.05）；위암조직급외주혈중적CDX2기인계동자CpG도갑기화정정상관（r=0.5913，P=0.0012）。결론：위암조직급외주혈중CDX2기인계동자CpG도갑기화수평일치，검측환자외주혈CDX2기인계동자CpG도갑기화가위위암적림상진치제공료일개간편、쾌첩적도경。
Objective: To explore the relationship and clinical significance between methylation of CDX2 gene in gastric cancer tissues and corresponding peripheral blood. Methods: Postoperative gastric tissue and the corresponding peripheral blood were collected from 40 patients with gastric cancer, 12 cases of benign gastric lesions in the adjacent normal gastric tissues and 20 cases of healthy blood samples. Methylation specific PCR was performed to detect the methylation status of CDX2 in the tumor tissues and the paired peripheral blood from 40 gastric cancer patients, to analyze the clinical significance and the relationship of each other. Results:The positive rate of methylation of CDX2 gene promoter CpG island was 72.5%(29/40) in the tumor tissues and 62.5%(25/40) in serum of gastric cancer patients, and the difference had no statistical significance. Correlation analysis showed that the CpG island methylation status of CDX2 gene promoter in gastric cancer tissues and corresponding peripheral blood was positively correlated (r=0.5913,P=0.0012). Conclusion: CDX2 gene promoter CpG island methylation can be detected in a high proportion of the tumor tissues consistent with that in the serum of gastric cancer patients, and the detection of methylation status of CDX2 gene in peripheral blood provides a more simple and fast way for the medical treatment of gastric cancer.
