南京师大学报（自然科学版）
南京師大學報（自然科學版）
남경사대학보（자연과학판）
Journal of Nanjing Normal University (Natural Science Edition)
2015年
3期
14-24
,共11页
袁秀雪%陈恩懿%肖梦思%赵传峰%周家宏
袁秀雪%陳恩懿%肖夢思%趙傳峰%週傢宏
원수설%진은의%초몽사%조전봉%주가굉
竹红菌甲素%铂竹红菌甲素复合物%牛血红蛋白%相互作用
竹紅菌甲素%鉑竹紅菌甲素複閤物%牛血紅蛋白%相互作用
죽홍균갑소%박죽홍균갑소복합물%우혈홍단백%상호작용
hypocrellin A%platinum-hypocrellin A%bovine hemoglobin%interaction
通过紫外-可见吸收光谱、瞬态荧光光谱以及圆二色谱来比较光疗药物竹红菌甲素及铂竹红菌甲素复合物与牛血红蛋白的相互作用。结果表明，虽然竹红菌甲素及铂竹红菌甲素复合物与牛血红蛋白的反应机理都是基于电子转移的静态猝灭，但是铂竹红菌甲素复合物与牛血红蛋白的反应不仅要比竹红菌甲素强，而且反应后牛血红蛋白的微环境和构像的改变也要大于竹红菌甲素。
通過紫外-可見吸收光譜、瞬態熒光光譜以及圓二色譜來比較光療藥物竹紅菌甲素及鉑竹紅菌甲素複閤物與牛血紅蛋白的相互作用。結果錶明，雖然竹紅菌甲素及鉑竹紅菌甲素複閤物與牛血紅蛋白的反應機理都是基于電子轉移的靜態猝滅，但是鉑竹紅菌甲素複閤物與牛血紅蛋白的反應不僅要比竹紅菌甲素彊，而且反應後牛血紅蛋白的微環境和構像的改變也要大于竹紅菌甲素。
통과자외-가견흡수광보、순태형광광보이급원이색보래비교광료약물죽홍균갑소급박죽홍균갑소복합물여우혈홍단백적상호작용。결과표명，수연죽홍균갑소급박죽홍균갑소복합물여우혈홍단백적반응궤리도시기우전자전이적정태졸멸，단시박죽홍균갑소복합물여우혈홍단백적반응불부요비죽홍균갑소강，이차반응후우혈홍단백적미배경화구상적개변야요대우죽홍균갑소。
The interaction mechanisms of Hypocrellin A(HA)and its platinum complex(Pt-HA)with bovine hemoglobin (BHb)were compared by ultraviolet-visible(UV-Vis)absorption,time-resolved fluorescence spectra,circular dichroism (CD)and induced circular dichroism(ICD)spectra,respectively. The UV-Vis results revealed that both HA and Pt-HA could interact with BHb and the latter interaction was stronger than HA. In addition,quenching mechanism of HA and Pt-HA were both static process,which were ascribed to the electron transfer. Moreover,CD and ICD spectrum revealed that the microenvironment and conformation of BHb were changed after conjugation with HA and Pt-HA,as the latter al?tered more acute. Finally,the comparison results demonstrated that the interaction of Pt-HA and BHb was stronger and more stable than HA.
