CAJ | 학술논문

目的：本文旨在介绍大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）体外培养体系建立的一套完整方法，及操作中的相关注意事项。方法体重100g 左右健康清洁 SD 大鼠，取双侧股骨及胫骨，冲出骨髓腔细胞后培养，72h 后全量换液，待细胞融合至80％左右时传代培养，取生长良好的 P3代细胞通过流式细胞仪，进行 CD90、CD45表型鉴定。结果体外培养 P3代细胞表面标记物 CD90、CD45鉴定完全符合标准，BMSCs 纯化并大量扩增。结论通过全骨髓贴壁培养法可以建立一套完整的体外培养体系。
목적：본문지재개소대서골수간충질간세포（bone marrow mesenchymal stem cells，BMSCs）체외배양체계건립적일투완정방법，급조작중적상관주의사항。방법체중100g 좌우건강청길 SD 대서，취쌍측고골급경골，충출골수강세포후배양，72h 후전량환액，대세포융합지80％좌우시전대배양，취생장량호적 P3대세포통과류식세포의，진행 CD90、CD45표형감정。결과체외배양 P3대세포표면표기물 CD90、CD45감정완전부합표준，BMSCs 순화병대량확증。결론통과전골수첩벽배양법가이건립일투완정적체외배양체계。
Objective To introduce a complete set of methods for the establishment of rat bone marrow mesen-chymal stem cells in vitro culture system and the relevant matters needing attention in the operation.Methods We selected healthy clean SD rats about 100g,cut out the bilateral femur and tibia,and developed the cells out of the bone marrow cavity.The cell culture fluid was all changed in 72 hours.When the cell fusion grew to 80%,we used trypsin for digestion and passaging the culture.The expression of CD90 and CD45 in P3 cells was identified by flow cytometry.Results The cell surface antigens CD90 and CD45 of in vitro cultured P3 cells were all in line with the standard.BMSCs were purified and amplified in vitro.Conclusion A complete set of in vitro culture sys-tem was established by the method of whole bone marrow adherent culture.