北京大学学报（医学版）
北京大學學報（醫學版）
북경대학학보（의학판）
Journal of Peking University (Health Sciences)
2015年
5期
820-824
,共5页
冯向辉%张立%徐莉%孟焕新%陈智滨%释栋%路瑞芳
馮嚮輝%張立%徐莉%孟煥新%陳智濱%釋棟%路瑞芳
풍향휘%장립%서리%맹환신%진지빈%석동%로서방
侵袭性牙周炎%伴放线聚集杆菌%血清组%免疫球蛋白G
侵襲性牙週炎%伴放線聚集桿菌%血清組%免疫毬蛋白G
침습성아주염%반방선취집간균%혈청조%면역구단백G
Aggressive periodontitis%Aggregatibacter actinomycetemcomitans%Serogroup%Immuno-globulin G
目的：分析侵袭性牙周炎（aggressive periodontitis， AgP）患者血清中的抗伴放线聚集杆菌（Aggregatibacter actinomycetemcomitans， Aa）血清c型IgG抗体滴度及其影响因素。方法：采集62例AgP患者（18例切磨牙型，44例广泛型）和45例牙周健康者的空腹静脉血，同时收集AgP患者的龈下菌斑和非刺激性全唾液用于Aa的检测（ PCR方法），应用酶联免疫吸附方法测定血清中抗Aa血清c型IgG抗体滴度。结果：AgP组和健康对照者血清中的抗Aa血清c型IgG抗体检出率均为100％， AgP 组的抗体滴度为11．1±1．9，显著高于健康对照组（9．1±1．8， P＜0．01）。切磨牙型AgP患者的抗Aa血清c型的IgG抗体滴度和抗体升高率与广泛型比较差异无统计学意义。唾液或龈下菌斑样本 Aa 检出阳性的 AgP 患者抗体滴度显著高于Aa 阴性患者（11．9±1．3 vs．10．7±2．1， P＜0．05）。结论：Aa血清c型为我国AgP患者定植的Aa的重要血清型，广泛型AgP患者血清抗Aa抗体滴度与切磨牙型AgP患者无明显差异。
目的：分析侵襲性牙週炎（aggressive periodontitis， AgP）患者血清中的抗伴放線聚集桿菌（Aggregatibacter actinomycetemcomitans， Aa）血清c型IgG抗體滴度及其影響因素。方法：採集62例AgP患者（18例切磨牙型，44例廣汎型）和45例牙週健康者的空腹靜脈血，同時收集AgP患者的齦下菌斑和非刺激性全唾液用于Aa的檢測（ PCR方法），應用酶聯免疫吸附方法測定血清中抗Aa血清c型IgG抗體滴度。結果：AgP組和健康對照者血清中的抗Aa血清c型IgG抗體檢齣率均為100％， AgP 組的抗體滴度為11．1±1．9，顯著高于健康對照組（9．1±1．8， P＜0．01）。切磨牙型AgP患者的抗Aa血清c型的IgG抗體滴度和抗體升高率與廣汎型比較差異無統計學意義。唾液或齦下菌斑樣本 Aa 檢齣暘性的 AgP 患者抗體滴度顯著高于Aa 陰性患者（11．9±1．3 vs．10．7±2．1， P＜0．05）。結論：Aa血清c型為我國AgP患者定植的Aa的重要血清型，廣汎型AgP患者血清抗Aa抗體滴度與切磨牙型AgP患者無明顯差異。
목적：분석침습성아주염（aggressive periodontitis， AgP）환자혈청중적항반방선취집간균（Aggregatibacter actinomycetemcomitans， Aa）혈청c형IgG항체적도급기영향인소。방법：채집62례AgP환자（18례절마아형，44례엄범형）화45례아주건강자적공복정맥혈，동시수집AgP환자적간하균반화비자격성전타액용우Aa적검측（ PCR방법），응용매련면역흡부방법측정혈청중항Aa혈청c형IgG항체적도。결과：AgP조화건강대조자혈청중적항Aa혈청c형IgG항체검출솔균위100％， AgP 조적항체적도위11．1±1．9，현저고우건강대조조（9．1±1．8， P＜0．01）。절마아형AgP환자적항Aa혈청c형적IgG항체적도화항체승고솔여엄범형비교차이무통계학의의。타액혹간하균반양본 Aa 검출양성적 AgP 환자항체적도현저고우Aa 음성환자（11．9±1．3 vs．10．7±2．1， P＜0．05）。결론：Aa혈청c형위아국AgP환자정식적Aa적중요혈청형，엄범형AgP환자혈청항Aa항체적도여절마아형AgP환자무명현차이。
Objective:To analyze the serum IgG titers to Aggregatibacter actinomycetemcomitans ( Aa ) and associated factors in patients with aggressive periodontitis (AgP).Methods:Venous blood samples were collected from 62 AgP patients and 45 periodontal healthy controls , unstimulated whole saliva and pooled subgingival plaque samples of AgP patients were also collected for the detection of Aa ( PCR method) .Serum IgG titers to Aa serotype c were measured by enzyme-linked immunosorbnent assay ( ELISA) .Results:The detection rates of serum IgG to Aa serotype c in the AgP patients and the healthy controls were both 100%.The AgP patients exhibited significantly higher IgG titers to Aa serotype c than the healthy controls (11.1 ±1.9 vs.9.1 ±1.8, P<0.01).There was no significant difference in serum IgG levels to Aa serotype c and in the prevalence of high-responding patients to Aa serotype c between the incisor-first molar type AgP patients and generalized AgP patients .Serum IgG titers to Aa serotype c in the Aa-positive AgP patients ( the patients who were Aa-positive in subgingival plaque or saliva ) were sig-nificantly higher than those of the Aa-negative patients (11.9 ±1.3 vs.10.7 ±2.1, P<0.05).Con-clusion:Serotype c was the main serotype of Aa in Chinese patients with AgP .Serum IgG responses in generalized AgP patients were comparable to those in incisor-first molar type AgP patients .
