CAJ | 학술논문

目的探讨miR-29c-5p在胆囊癌组织中的表达情况及其临床意义.方法采用实时定量PCR技术(stem-loop real-time reverse transcription-polymerase chain reaction,qRT-PCR)检测40例胆囊癌组织与癌旁组织中miR-29c-5p的相对表达量;分析miR-29c-5p表达与胆囊癌患者临床病理因素及预后的关系.结果与癌旁组织相比,胆囊癌组织中miR-29c-5p表达明显降低(Z=-2.521,P =0.008),其表达与胆囊癌患者的淋巴结转移有关(x2=15.140,P＜0.001),而与年龄、性别、肿瘤大小、分化程度、浸润深度及肿瘤TNM分期无关(均P＞0.05).MiR-29c-5p低表达与高表达的患者中位生存率分别为7.2个月和20.2个月,1年及3年生存率分别为32.0％、16.0％和55.7％、33.4％,差异有统计学意义(P=0.040).MiR-29c-5p表达量不是影响胆囊癌患者生存的独立风险因素(P =0.974).结论 MiR-29c-5p在胆囊癌组织中表达下调,并可能与胆囊癌的淋巴结转移有关;miR-29c-5p低表达提示预后不良.
목적 탐토miR-29c-5p재담낭암조직중적표체정황급기림상의의.방법 채용실시정량PCR기술(stem-loop real-time reverse transcription-polymerase chain reaction,qRT-PCR)검측40례담낭암조직여암방조직중miR-29c-5p적상대표체량;분석miR-29c-5p표체여담낭암환자림상병리인소급예후적관계.결과 여암방조직상비,담낭암조직중miR-29c-5p표체명현강저(Z=-2.521,P =0.008),기표체여담낭암환자적림파결전이유관(x2=15.140,P＜0.001),이여년령、성별、종류대소、분화정도、침윤심도급종류TNM분기무관(균P＞0.05).MiR-29c-5p저표체여고표체적환자중위생존솔분별위7.2개월화20.2개월,1년급3년생존솔분별위32.0％、16.0％화55.7％、33.4％,차이유통계학의의(P=0.040).MiR-29c-5p표체량불시영향담낭암환자생존적독립풍험인소(P =0.974).결론 MiR-29c-5p재담낭암조직중표체하조,병가능여담낭암적림파결전이유관;miR-29c-5p저표체제시예후불량.
Objective To investigate the expression of miR-29c-5p in patients with gallbladder cancer (GBC) and to analyze the association of the expression with the clinicopathologic characteristics.Methods The expression levels of miR-29c-5p in 40 patients with GBC along with their matched adjacent tissues were detected by using stem-loop real-time reverse transcription-polymerase chain reaction (qRT-qPCR).The relations of miR-29c-5p expression with the clinicopathologic factors and prognosis of GBC patients were analyzed.Results Compared with tumor-adjacent tissue,the miR-29c-5p expression in GBC was significantly downregulated (Z =-2.521,P =0.008).The expression of miR-29c-5p was closely associated with lynphatic metastasis (x2 =15.140,P ＜ 0.001),but irrelevant to age,sex,tumor size,differentiation,T stage and TNM stage (all P ＞ 0.05).The average survival period of low and high expression of miR-29c-5p were 7.2 months and 20.2 months.The one-year and three-year survival rate of patients with low expression of miR-29c-5p were significantly lower than those with high levels (32.0％,16.0％ vs 55.7％,33.4％) (P =0.040).Low miR-29c-5p expression is not risk factor affecting the survival of GBC patients (P =0.974).Conclusions The miR-29c-5p expression is downregnlated in GBC tissue,which probably may be associated with the metastasis of GBC,and low miR-29c-5p expression may predict an unfavorable outcome.