北京大学学报(医学版)
北京大學學報(醫學版)
북경대학학보(의학판)
Journal of Peking University (Health Sciences)
2015年
5期
814-819
,共6页
余涛%姜婷%魏青梅%李宜芬%David L.Kaplan
餘濤%薑婷%魏青梅%李宜芬%David L.Kaplan
여도%강정%위청매%리의분%David L.Kaplan
骨形态发生蛋白2%脂多糖类%牙髓炎%牙髓覆盖术%丝素蛋白质类
骨形態髮生蛋白2%脂多糖類%牙髓炎%牙髓覆蓋術%絲素蛋白質類
골형태발생단백2%지다당류%아수염%아수복개술%사소단백질류
Bone morphogenetic protein 2%Lipopolysaccharides%Pulpitis%Dental pulp capping%Fi-broins
目的:探讨将丝蛋白复合骨形态发生蛋白2(bone morphogenetic protein 2, BMP-2)作为盖髓剂,治疗由脂多糖(lipopolysaccharide, LPS)引起的大鼠炎性牙髓的效果。方法:30只雄性Wistar大鼠,随机分为5组:(1)未手术正常对照组;(2)空白对照组(无盖髓剂);(3)氢氧化钙组;(4)静电纺丝丝蛋白组(简称丝蛋白组);(5)静电纺丝丝蛋白复合BMP-2组(简称丝蛋白复合BMP-2组)。将第2~5组双侧上颌第一磨牙开髓并放置LPS,不使用盖髓剂或使用相应盖髓剂,然后封闭开髓孔。所有组于术后第3、7、14天制作石蜡切片,行HE染色及CD14免疫组织化学染色,采用image-pro plus ( IPP)软件测量免疫组织化学染色的光密度( D)值。结果:HE染色结果显示丝蛋白复合BMP-2组在各盖髓组中,术后7及14 d炎性细胞分级最低,其次是氢氧化钙组及丝蛋白组,空白对照组最高;修复性牙本质形成分级的高低顺序则相反。 CD14免疫组织化学染色结果显示术后3及7 d,使用盖髓剂的3个组之间D值差异无统计学意义,但均显著低于空白对照组;14 d时丝蛋白复合BMP-2组D值(0.145±0.011)显著低于空白对照(0.287±0.019)、氢氧化钙(0.170±0.017)、丝蛋白(0.175±0.018)3个组(P<0.05)。结论:丝蛋白复合BMP-2应用于炎性牙髓的盖髓,能减轻炎症,诱导修复性牙本质的形成,提高对炎性牙髓的治疗效果。
目的:探討將絲蛋白複閤骨形態髮生蛋白2(bone morphogenetic protein 2, BMP-2)作為蓋髓劑,治療由脂多糖(lipopolysaccharide, LPS)引起的大鼠炎性牙髓的效果。方法:30隻雄性Wistar大鼠,隨機分為5組:(1)未手術正常對照組;(2)空白對照組(無蓋髓劑);(3)氫氧化鈣組;(4)靜電紡絲絲蛋白組(簡稱絲蛋白組);(5)靜電紡絲絲蛋白複閤BMP-2組(簡稱絲蛋白複閤BMP-2組)。將第2~5組雙側上頜第一磨牙開髓併放置LPS,不使用蓋髓劑或使用相應蓋髓劑,然後封閉開髓孔。所有組于術後第3、7、14天製作石蠟切片,行HE染色及CD14免疫組織化學染色,採用image-pro plus ( IPP)軟件測量免疫組織化學染色的光密度( D)值。結果:HE染色結果顯示絲蛋白複閤BMP-2組在各蓋髓組中,術後7及14 d炎性細胞分級最低,其次是氫氧化鈣組及絲蛋白組,空白對照組最高;脩複性牙本質形成分級的高低順序則相反。 CD14免疫組織化學染色結果顯示術後3及7 d,使用蓋髓劑的3箇組之間D值差異無統計學意義,但均顯著低于空白對照組;14 d時絲蛋白複閤BMP-2組D值(0.145±0.011)顯著低于空白對照(0.287±0.019)、氫氧化鈣(0.170±0.017)、絲蛋白(0.175±0.018)3箇組(P<0.05)。結論:絲蛋白複閤BMP-2應用于炎性牙髓的蓋髓,能減輕炎癥,誘導脩複性牙本質的形成,提高對炎性牙髓的治療效果。
목적:탐토장사단백복합골형태발생단백2(bone morphogenetic protein 2, BMP-2)작위개수제,치료유지다당(lipopolysaccharide, LPS)인기적대서염성아수적효과。방법:30지웅성Wistar대서,수궤분위5조:(1)미수술정상대조조;(2)공백대조조(무개수제);(3)경양화개조;(4)정전방사사단백조(간칭사단백조);(5)정전방사사단백복합BMP-2조(간칭사단백복합BMP-2조)。장제2~5조쌍측상합제일마아개수병방치LPS,불사용개수제혹사용상응개수제,연후봉폐개수공。소유조우술후제3、7、14천제작석사절편,행HE염색급CD14면역조직화학염색,채용image-pro plus ( IPP)연건측량면역조직화학염색적광밀도( D)치。결과:HE염색결과현시사단백복합BMP-2조재각개수조중,술후7급14 d염성세포분급최저,기차시경양화개조급사단백조,공백대조조최고;수복성아본질형성분급적고저순서칙상반。 CD14면역조직화학염색결과현시술후3급7 d,사용개수제적3개조지간D치차이무통계학의의,단균현저저우공백대조조;14 d시사단백복합BMP-2조D치(0.145±0.011)현저저우공백대조(0.287±0.019)、경양화개(0.170±0.017)、사단백(0.175±0.018)3개조(P<0.05)。결론:사단백복합BMP-2응용우염성아수적개수,능감경염증,유도수복성아본질적형성,제고대염성아수적치료효과。
Objective:To identify the healing effect of electrospun silk fibroin-BMP-2 as a biologic pulp capping agent to inflammatory pulp in rat caused by lipopolysaccharide ( LPS) .Methods:A total of 30 healthy adult male Wistar rats were randomly divided into five groups:(1) normal control group without operation;(2) blank control group without capping agents;(3) calcium hydroxide capping group;(4) electrospun silk fibroin capping group;(5) electrospun silk fibroin-BMP-2 capping group .Bilateral up-per first molars of each rat in group 2-5 were drilled to expose the pulp to LPS which was used to estab-lish a model of inflammatory pulp .The exposed pulp was capped with different capping agents or without capping agents.Then the hole was sealed.The animals were sacrificed on days 3, 7, and 14 post-opera-tion and histological analysis was carried out , including HE stain and CD 14 immunohistochemical stain . Results:On day 7 and 14 , the lowest inflammatory reaction score in HE stain among pulp capping groups was that of silk fibroin-BMP-2 group .The next were calcium hydroxide group and silk fibroin group .That of blank control group was the highest .The ranking of reparative dentine scores of those groups was just reversed.The D values of immunohistochemical stain of CD 14 were not significantly different in groups applied pulp capping agents but significantly lower than blank control group on days 3 and 7.However, the D value of silk fibroin-BMP-2 group ( 0 .145 ±0 .011 ) was significantly lower than blank control group (0.287 ±0.019), calcium hydroxide group (0.170 ±0.017) and silk fibroin group (0.175 ± 0 .018 ) on day 14 .Conclusion:Electrospun silk fibroin compounded with BMP-2 promoted wound hea-ling of exposed pulp and had better potential to stimulate formation of reparative dentine to establish a suitable environment for pulp recovery .