CAJ | 학술논문

采用半制备液相色谱法制备N-乙酰基神经氨酸标准物质。分别以UV，IR，MS和NMR方法对N-乙酰基神经氨酸标准物质进行定性分析，以高效液相色谱方法进行均匀性检验和稳定性检验，经F检验表明在置信区间为95%的范围内测定结果稳定。与国内8家资质合格的实验室进行联合定值，计算出不确定度结果。N-乙酰基神经氨酸标准物质的定值结果为98.63%，置信水平为95%时的扩展不确定度为0.0986%(k=2)。该N-乙酰基神经氨酸标准物质可用于有关食品检测方法的校正。
채용반제비액상색보법제비N-을선기신경안산표준물질。분별이UV，IR，MS화NMR방법대N-을선기신경안산표준물질진행정성분석，이고효액상색보방법진행균균성검험화은정성검험，경F검험표명재치신구간위95%적범위내측정결과은정。여국내8가자질합격적실험실진행연합정치，계산출불학정도결과。N-을선기신경안산표준물질적정치결과위98.63%，치신수평위95%시적확전불학정도위0.0986%(k=2)。해N-을선기신경안산표준물질가용우유관식품검측방법적교정。
N-acetylneuraminic acid certified reference material was separated by semi-preparation HPLC. The qualitative analysis ofN-acetylneuraminic acid was identified by UV, IR, MS and NMR spectrum. The inspection of uniformity and stability were carried out by HPLC. The results examined byF-test statistical methods were stable in the confidence interval of 95%. The combined measurement was executed with 8 qualified laboratory rooms and the uncertainty value was counted at the same time. The purity ofN-acetylneuraminic acid certified reference material was 98.63% with the expanded uncertainty of 0.098 6%(k=2) in theconfidence interval of 95%.N-acetylneuraminic acid certified reference material can be used for food detection methods of calibration.