CAJ | 학술논문

目的：观察2‐（2‐巯基乙醇）‐3‐甲基‐1，4‐萘醌（Compound 5，Cpd5）对人宫颈癌 HeLa细胞增殖和凋亡的影响。方法四甲基偶氮唑蓝（M T T ）法观察Cpd5对HeLa细胞的生长抑制作用，Annexin V／PI双标记流式细胞术检测Cpd5对 HeLa细胞的凋亡诱导，Hoechst‐33258染色荧光显微镜观察细胞凋亡形态。结果10、20、30、40、50μmol／L Cpd5处理 HeLa细胞48 h ，生长抑制率分别为4.23、13.11、35.13、51.37、79.90％。不同时间点（12、24、48和72 h ）检测，细胞生长抑制率存在剂量‐时间依赖关系。流式细胞术检测，30μmol／L Cpd5处理12、24和48 h后，细胞凋亡率分别达7.15、16.07、44.16％；50μmol／L组的细胞凋亡率明显高于30μmol／L ，且随时间增加而显著增加。结论 Cpd5能以剂量－时间依赖的方式抑制HeLa细胞增殖并诱导调亡，是潜在的抗宫颈癌新药。
목적：관찰2‐（2‐구기을순）‐3‐갑기‐1，4‐내곤（Compound 5，Cpd5）대인궁경암 HeLa세포증식화조망적영향。방법사갑기우담서람（M T T ）법관찰Cpd5대HeLa세포적생장억제작용，Annexin V／PI쌍표기류식세포술검측Cpd5대 HeLa세포적조망유도，Hoechst‐33258염색형광현미경관찰세포조망형태。결과10、20、30、40、50μmol／L Cpd5처리 HeLa세포48 h ，생장억제솔분별위4.23、13.11、35.13、51.37、79.90％。불동시간점（12、24、48화72 h ）검측，세포생장억제솔존재제량‐시간의뢰관계。류식세포술검측，30μmol／L Cpd5처리12、24화48 h후，세포조망솔분별체7.15、16.07、44.16％；50μmol／L조적세포조망솔명현고우30μmol／L ，차수시간증가이현저증가。결론 Cpd5능이제량－시간의뢰적방식억제HeLa세포증식병유도조망，시잠재적항궁경암신약。
Objective To investigate the influence of 2‐(2‐mercaptoethanol)‐3‐methyl‐1 ,4‐ naphthoquinone (Compound 5 , Cpd5) on human cervical cancer HeLa cells'apoptosis and proliferation .Methods MTT assay was used to test growth inhibi‐tion of HeLa by Cpd5 .An AnnexinV/PI kit was employed for quantifying apoptotic cells by flow cytometry .Moreover ,Ho‐echst‐33258 staining was applied to identify the apoptotic cells .Results Treated by Cpd5 at the concentrations of 10 ,20 ,30 , 40and 50 μmol/L for 48 h ,the ratios of the cells growth inhibition were respectively 4.23 ,13.11 ,35.13 ,51.37 ,79.90% .The proliferation inhibition was investigated after treatment by Cpd 5 at different concentrations for 12 ,24 ,48 and 72 h .The cells were inhibited by Cpd5 in a dose‐time‐dependent manner .Analyzed by flow cytometry ,the apoptotic cells were respectively 9.25 ,20.07 and 56.16% after treatment by 30μmol/L Cpd5 for 12 ,24 and 48 hours .The ratio of apoptotic cells in 50μmol/L group was significantly higher than that in 30μmol/L group .Conclusion Cpd5 inhibits HeLa cells'proliferation and induces ap‐optosis in a dose‐time‐dependent manner .Cpd5 is a novel potential anti cervical cancer drug .