CAJ | 학술논문

目的：探讨化学发光（CLIA）法与梅毒螺旋体抗体明胶颗粒凝集试验（TPPA）对梅毒螺旋体抗体检测的应用价值。方法采用CLIA法对患者血清进行梅毒螺旋体抗体检测，收集阳性血清标本425例，阴性血清标本90例，用T PPA进行确认；根据测定S／CO值将标本分为4组，统计各组CLIA法与T PPA的符合率。结果根据CLIA法检测结果，将标本分为4组：A 组（S／CO＜1．20，n＝90）；B组（S／CO为1．20～10．00，n＝139）；C组（S／CO＞10．00～20．88，n＝124）；D组（S／CO＞20．88，n＝162）。A ～D组 CLIA 法与 TPPA 的检测符合率分别为100．0％、68．3％、92．7％、100．0％，B组的检测符合率最低，与其他3组比较差异有统计学意义（ P＜0．05）。结论CLIA法检测梅毒具有敏感性高，自动化程度高，适合大批量标本常规筛查，CLIA法检测阳性结果需进一步做TP‐PA进行确证。
목적：탐토화학발광（CLIA）법여매독라선체항체명효과립응집시험（TPPA）대매독라선체항체검측적응용개치。방법채용CLIA법대환자혈청진행매독라선체항체검측，수집양성혈청표본425례，음성혈청표본90례，용T PPA진행학인；근거측정S／CO치장표본분위4조，통계각조CLIA법여T PPA적부합솔。결과근거CLIA법검측결과，장표본분위4조：A 조（S／CO＜1．20，n＝90）；B조（S／CO위1．20～10．00，n＝139）；C조（S／CO＞10．00～20．88，n＝124）；D조（S／CO＞20．88，n＝162）。A ～D조 CLIA 법여 TPPA 적검측부합솔분별위100．0％、68．3％、92．7％、100．0％，B조적검측부합솔최저，여기타3조비교차이유통계학의의（ P＜0．05）。결론CLIA법검측매독구유민감성고，자동화정도고，괄합대비량표본상규사사，CLIA법검측양성결과수진일보주TP‐PA진행학증。
Objective To explore the application value of Treponema pallidum antibody detection by combining CLIA and TPPA .Methods Treponema pallidum antibodies in serum were detected by CLIA ,and a total of 425 posi‐tive samples and 90 negative samples were collected ;then the collected samples were confirmed by TPPA .According to the S/CO values ,the samples were divided into four groups and the coincidence rate of CLIA and TPPA in each group was analyzed .Results According to the CLIA results ,the samples were divided into 4 groups:group A (S/CO<1 .20 ,n=90) ,group B (S/CO 1 .20-10 .00 ,n=139) ,group C (S/CO>10 .00 -20 .88 ,n=124) ,group D (S/CO>20 .88 ,n=162) .The coincidence rates of CLIA and TPPA in group A ,B ,C ,and D were 100 .0% ,68 .3% , 92 .7% ,and 100 .0% ,respectively .The coincidence rate in group B was the lowest ,which was significantly different from the other three groups (P<0 .05) .Conclusion CLIA is a highly sensitive and automated method ,which is suitable for clinical routine screening of a large number of specimens .The positive results of CLIA should be confirmed by TPPA .