CAJ | 학술논문

研究了小檗碱和溴酚蓝相互作用的荧光光谱,小檗碱能使溴酚蓝的荧光发生猝灭,猝灭强度随小檗碱浓度改变发生线性变化,相关系数为0.9998,线性范围为0.207~26μg·mL-1,检出限为0.062μg·mL-1,运用于血清和复方黄连素片小檗碱的测定,结果让人满意。据此提出了一种测定小檗碱的新方法。
연구료소벽감화추분람상호작용적형광광보,소벽감능사추분람적형광발생졸멸,졸멸강도수소벽감농도개변발생선성변화,상관계수위0.9998,선성범위위0.207~26μg·mL-1,검출한위0.062μg·mL-1,운용우혈청화복방황련소편소벽감적측정,결과양인만의。거차제출료일충측정소벽감적신방법。
The berberine and bromophenol blue’s fluorescence spectra interactions were discussed, bromophenol blue made berberine fluorescence quenching occurs, the quenching intensity with berberine concentration changed varies linearly correlation coefficient of 0. 9998 , linear range of 0. 207~26 μg · mL-1 , a detection limit of 0. 062 μg · mL-1 , used in serum and determination of compound berberine berberine results satisfactory. A new method for the determination of berberine was proposed.