北京大学学报（医学版）
北京大學學報（醫學版）
북경대학학보（의학판）
Journal of Peking University (Health Sciences)
2015年
5期
761-768
,共8页
张静%任景怡%陈红%韩冠平
張靜%任景怡%陳紅%韓冠平
장정%임경이%진홍%한관평
心绞痛,不稳定型%微RNAs%他汀类药物%血浆
心絞痛,不穩定型%微RNAs%他汀類藥物%血漿
심교통,불은정형%미RNAs%타정류약물%혈장
Angina,unstable%MicroRNAs%Statins%Plasma
目的：评估服用他汀类药物对不稳定性心绞痛患者血浆中microRNAs（ miRNAs）表达谱的影响。方法：采用miRNAs TLDA（Taqman low-density array）芯片对长期规律服用他汀类药物的不稳定性心绞痛患者（他汀治疗组， n＝6）及未曾服用过他汀类药物的不稳定性心绞痛患者（非他汀治疗组，n＝6）血浆中差异表达的miRNAs表达谱进行筛查，并选择部分差异表达的炎症相关的miRNAs在非心源性胸痛患者（对照组，n＝20）、未曾服用过他汀类药物的不稳定性心绞痛患者（非他汀治疗组，n＝26）、长期规律服用他汀类药物的不稳定性心绞痛患者（他汀治疗组，n＝19）中采用real-time PCR的方法进行验证。结果：他汀治疗组患者血浆中miRNAs的表达谱与非他汀治疗组患者相比差异有统计学意义，包括miR-106b、miR-21、miR-25、miR-451以及 miR-92a等炎症相关miRNAs在内的21种血浆miRNAs水平显著降低（组间差异倍数＞3，错误发现率＜0．0001％），选取miR-106b、miR-21、miR-25、miR-451以及miR-92a进行real-time PCR验证的结果与芯片筛查结果一致，与未曾服用过他汀类药物的患者相比，长期规律服用他汀类药物可降低上述5种炎症相关miRNAs的表达水平（P＜0．05），而非他汀治疗组患者血浆中上述5种炎症相关miRNAs的表达水平高于对照组（P＜0．001）。结论：服用他汀类药物可显著降低不稳定性心绞痛患者血浆中一组炎症相关的miRNAs的表达水平。
目的：評估服用他汀類藥物對不穩定性心絞痛患者血漿中microRNAs（ miRNAs）錶達譜的影響。方法：採用miRNAs TLDA（Taqman low-density array）芯片對長期規律服用他汀類藥物的不穩定性心絞痛患者（他汀治療組， n＝6）及未曾服用過他汀類藥物的不穩定性心絞痛患者（非他汀治療組，n＝6）血漿中差異錶達的miRNAs錶達譜進行篩查，併選擇部分差異錶達的炎癥相關的miRNAs在非心源性胸痛患者（對照組，n＝20）、未曾服用過他汀類藥物的不穩定性心絞痛患者（非他汀治療組，n＝26）、長期規律服用他汀類藥物的不穩定性心絞痛患者（他汀治療組，n＝19）中採用real-time PCR的方法進行驗證。結果：他汀治療組患者血漿中miRNAs的錶達譜與非他汀治療組患者相比差異有統計學意義，包括miR-106b、miR-21、miR-25、miR-451以及 miR-92a等炎癥相關miRNAs在內的21種血漿miRNAs水平顯著降低（組間差異倍數＞3，錯誤髮現率＜0．0001％），選取miR-106b、miR-21、miR-25、miR-451以及miR-92a進行real-time PCR驗證的結果與芯片篩查結果一緻，與未曾服用過他汀類藥物的患者相比，長期規律服用他汀類藥物可降低上述5種炎癥相關miRNAs的錶達水平（P＜0．05），而非他汀治療組患者血漿中上述5種炎癥相關miRNAs的錶達水平高于對照組（P＜0．001）。結論：服用他汀類藥物可顯著降低不穩定性心絞痛患者血漿中一組炎癥相關的miRNAs的錶達水平。
목적：평고복용타정류약물대불은정성심교통환자혈장중microRNAs（ miRNAs）표체보적영향。방법：채용miRNAs TLDA（Taqman low-density array）심편대장기규률복용타정류약물적불은정성심교통환자（타정치료조， n＝6）급미증복용과타정류약물적불은정성심교통환자（비타정치료조，n＝6）혈장중차이표체적miRNAs표체보진행사사，병선택부분차이표체적염증상관적miRNAs재비심원성흉통환자（대조조，n＝20）、미증복용과타정류약물적불은정성심교통환자（비타정치료조，n＝26）、장기규률복용타정류약물적불은정성심교통환자（타정치료조，n＝19）중채용real-time PCR적방법진행험증。결과：타정치료조환자혈장중miRNAs적표체보여비타정치료조환자상비차이유통계학의의，포괄miR-106b、miR-21、miR-25、miR-451이급 miR-92a등염증상관miRNAs재내적21충혈장miRNAs수평현저강저（조간차이배수＞3，착오발현솔＜0．0001％），선취miR-106b、miR-21、miR-25、miR-451이급miR-92a진행real-time PCR험증적결과여심편사사결과일치，여미증복용과타정류약물적환자상비，장기규률복용타정류약물가강저상술5충염증상관miRNAs적표체수평（P＜0．05），이비타정치료조환자혈장중상술5충염증상관miRNAs적표체수평고우대조조（P＜0．001）。결론：복용타정류약물가현저강저불은정성심교통환자혈장중일조염증상관적miRNAs적표체수평。
Objective:To explore the influence of treatment with HMG-CoA reductase inhibitors ( sta-tins) on the expression profile of microRNAs ( miRNAs) in the plasma of patients with unstable angina ( UA) .Methods:The Taqman low-density miRNA array ( TLDA) and significance analysis of microar-rays ( SAM) were used to identify distinct miRNA expression profiles in the plasma of UA patients treated with long-term and regular statins ( UA receiving statins , n=6 ) compared with UA patients who had not received statins therapy before ( UA received no statins , n=6 ) .These differentially expressed miRNAs discovered in the profiling were further validated by real-time PCR in another 20 controls with non-cardiac chest pain , 26 UA patients received no statins , and 19 UA patients received statins .Results: By using TLDA and SAM , significantly decreased expression levels of 21 miRNAs were observed in the UA pa-tients receiving statins compared with those who received no statins ( fold change >3 and false discovery rate<0 .0001%) .The unsupervised hierarchical clustering based on miRNA expression clearly separa-ted the UA patients receiving statins from those who received no statins .Consistent with the profiling da-ta, the levels of 5 inflammation-associated miRNAs (miR-106b, miR-21, miR-25, miR-451, and miR-92a) were down regulated (P<0.05) in the UA patients receiving statins compared with those who re-ceived no statins.Conclusion: A group of inflammation-associated miRNAs, consisting of miR-106b, miR-21, miR-25, miR-451, and miR-92a, could be decreased by treatment with statins and may be used as a novel biomarker for effectiveness of statins therapy in patients with UA .
