CAJ | 학술논문

背景：代谢综合征对人体的危害极大，且受到多种因素的影响。通过构建饮食诱导动物模型，可以更好的分析营养因素和代谢综合征之间的关系，为临床治疗等提供可靠的参考依据。目的：构建高脂饮食喂养肥胖小鼠模型，探讨营养因素和代谢综合征的关系。方法：选择30只小鼠随机分为模型组20只和对照组10只，分别予以高脂饮食纯化饲料和普通饲料喂养，连续喂养10周。结果与结论：与对照组相比，高脂饮食纯化饲料喂养后1周，模型组小鼠即出现体质量升高，且随着喂养时间增加呈现出差异逐渐增大的情况；喂养后8周，模型组的体质量指数和显著升高(P <0.05)。喂养后4周，模型组的空腹静脉全血血糖即显著升高，且随着喂养时间的增长呈现出逐渐上升的情况；喂养后5周，模型组的空腹胰岛素水平也开始出现升高；经口服糖耐量实验，随着喂养时间的增长，模型组小鼠表现出糖耐量逐渐下降的趋势；喂养后8周，模型组的血清总胆固醇、高密度脂蛋白胆固醇水平均出现显著性上升高(P <0.05)；喂养后10周，模型组的三酰甘油、血清总胆固醇、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇均升高(P <0.05)。结果证实，实验成功构建了高脂饮食喂养肥胖小鼠模型，可能模拟人类的代谢综合征的自然发病过程，且营养因素与代谢综合征密切相关。
배경：대사종합정대인체적위해겁대，차수도다충인소적영향。통과구건음식유도동물모형，가이경호적분석영양인소화대사종합정지간적관계，위림상치료등제공가고적삼고의거。목적：구건고지음식위양비반소서모형，탐토영양인소화대사종합정적관계。방법：선택30지소서수궤분위모형조20지화대조조10지，분별여이고지음식순화사료화보통사료위양，련속위양10주。결과여결론：여대조조상비，고지음식순화사료위양후1주，모형조소서즉출현체질량승고，차수착위양시간증가정현출차이축점증대적정황；위양후8주，모형조적체질량지수화현저승고(P <0.05)。위양후4주，모형조적공복정맥전혈혈당즉현저승고，차수착위양시간적증장정현출축점상승적정황；위양후5주，모형조적공복이도소수평야개시출현승고；경구복당내량실험，수착위양시간적증장，모형조소서표현출당내량축점하강적추세；위양후8주，모형조적혈청총담고순、고밀도지단백담고순수평균출현현저성상승고(P <0.05)；위양후10주，모형조적삼선감유、혈청총담고순、저밀도지단백담고순화고밀도지단백담고순균승고(P <0.05)。결과증실，실험성공구건료고지음식위양비반소서모형，가능모의인류적대사종합정적자연발병과정，차영양인소여대사종합정밀절상관。
BACKGROUND:Metabolic syndrome greatly harms the human body, and is affected by many factors. Through constructing diet-induced animal models, we can better analyze the relationship between nutritional factor and metabolic syndrome, and provide reliable references for the clinical treatment of this disorder. OBJECTIVE:To construct obese mouse models with high-fat diet feeding and discuss the relationship between nutritional factor and metabolic syndrome. METHODS:Thirty mice were selected and randomly divided into model group (n=20) and control group (n=10), and were fed with high-fat and normal animal feeds for 10 consecutive weeks. RESULTS AND CONCLUSION:Compared with the control group, after 1 week of feeding with high-fat animal feeds, body weight of mice in the model group raised, and differences gradualy increased with the feeding time increased. After 8 weeks of feeding, body mass index of mice in the model group significantly raised (P < 0.05). After 4 weeks of feeding, fasting venous blood glucose level of mice in the model group significantly raised, and showed a gradual rise trend with feeding time. After 5 weeks of feeding, fasting insulin level of mice in the model group also began to rise. The oral glucose tolerance test showed that mice in the model group showed a gradual downward trend of glucose tolerance with feeding time. After 8 weeks of feeding, serum levels of total cholesterol and high density lipoprotein cholesterol in the model group significantly raised (P < 0.05). After 10 weeks of feeding, serum levels of triacylglycerol, total cholesterol, low density lipoprotein cholesterol and high density lipoprotein cholesterol in the model group raised (P< 0.05). The results demonstrate that obese mouse models were successfuly constructed with high-fat diet feeding, which can simulate the natural progression of metabolic syndrome in human, moreover, the nutritional factor is closely related to metabolic syndrome.