CAJ | 학술논문

目的：探讨环介导等温扩增技术（LAMP）作为快速检测方法对金黄色葡萄球菌快速检测的可行性和可靠性。方法建立LAMP反应体系，优化反应条件，进行灵敏度和特异度试验。结果筛选出了LAMP反应检测金黄色葡萄球菌 nuc基因的最佳引物组，最佳反应温度为62℃，LAMP和PCR检测 nuc基因的最低检测限分别为1．47、14．7 pg／μL ，检测金黄色葡萄球菌的特异度为100％。结论 L A M P具有操作简单、敏感度高、特异性强的特点，能够满足基层实验室、应急检测或现场检测等方面的使用需求。
목적：탐토배개도등온확증기술（LAMP）작위쾌속검측방법대금황색포도구균쾌속검측적가행성화가고성。방법건립LAMP반응체계，우화반응조건，진행령민도화특이도시험。결과사선출료LAMP반응검측금황색포도구균 nuc기인적최가인물조，최가반응온도위62℃，LAMP화PCR검측 nuc기인적최저검측한분별위1．47、14．7 pg／μL ，검측금황색포도구균적특이도위100％。결론 L A M P구유조작간단、민감도고、특이성강적특점，능구만족기층실험실、응급검측혹현장검측등방면적사용수구。
Objective To investigate the feasibility and reliability of loop‐mediated isothermal amplification (LAMP) in rapid detection of Staphylococcus aureus as a rapid detection method .Methods LAMP reaction system was established ,the reaction conditions was optimized ,the sensitivity and specificity experiments were carried out . Results The best primer set was selected to detect staphylococcus aureus for nuc genes through LAMP reaction .The best reaction temperature selected was 62 ℃ ,and the lowest detectable limits of LAMP and PCR for nuc genes were 1 .47 ,14 .7 pg/μL respectively .The specificities of two methods to detect Staphylococcus aureus were 100% .Conclu‐sion LAMP detection method is simple ,safe operation ,with high sensitivity and high specificity ,which can meet the need of grassroots laboratory ,emergency detection and on‐site testing and other aspects .